User:Fermenter User/Notebook/DOE Workshop 20110405: Difference between revisions

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== Day 3 (April 7th, Thu) ==
== Day 9 (April 13th, Thu) ==


{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Runs'''
| align="center" style="background:#f0f0f0;"|'''Runs'''
| align="center" style="background:#f0f0f0;"|'''4, 5, 6'''
| align="center" style="background:#f0f0f0;"|'''16, 17, 18'''
| align="center" style="background:#f0f0f0;"|'''7, 8, 9'''
| align="center" style="background:#f0f0f0;"|'''19, 20'''
| align="center" style="background:#f0f0f0;"|'''10, 11, 12'''
| align="center" style="background:#f0f0f0;"|''''''
|-
|-
| 07:30||||<font color=gray> SH: Prepare hight density K19 cells </font>||
| 07:30||||<font color=gray> SH: Prepare hight density K19 cells </font>||
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| 09:00||<font color=red> Group A: Colony check </font>|| <font color=blue> Group B: Sampling </font>||
| 09:00||<font color=red> Group A: Colony check </font>|| <font color=blue> Group B: Sampling </font>||
|-
|-
| 09:30||||<font color=brown> Ann: Phage dilution </font>||<font color=red> Group A: Assemble bioreactors </font>
| 09:30||||<font color=brown> Ann: Phage dilution </font>||
|-
|-
| 09:50||||<font color=brown> Ann: OD of HD cells </font>||<font color=red> Group A: Autoclve</font>
| 09:50||||<font color=brown> Ann: OD of HD cells </font>||
|-
|-
| 10:00||||<font color=brown> Ann: Dry plates </font>||
| 10:00||||<font color=brown> Ann: Dry plates </font>||
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| 10:30||||<font color=brown> Ann: Infect K19 cells </font>||
| 10:30||||<font color=brown> Ann: Infect K19 cells </font>||
|-
|-
| 11:10||||<font color=brown> Ann: Spot titer </font>||<font color=red> Group A: Install bioreactors</font>
| 11:10||||<font color=brown> Ann: Spot titer </font>||
|-
|-
| 15:30||||||<font color=gray> SH: Thaw stock
| 15:30||||||<font color=gray>  
|-
|-
| 16:50||||||<font color=gray> SH: Pre-culture for HD K19 cells
| 16:50||||||<font color=gray>  
|-
|-
| 17:00||||||<font color=gray> SH: Inoculation
| 17:00||||||<font color=gray>  
|-
|-
|  
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  10:00 - 11:00 Presentation (Rm 4.303)
  10:00 - 11:00 Presentation (Rm 4.303)
   # DOE Step 1. Design Experiments
   # Case Studies: Publication


== Helful links ==
== Helful links ==

Revision as of 13:12, 22 March 2011

WORK B1


1st LSI-CBR Design of Experiment (DOE) process Optimization Workshop

Teaching:


When

April 5th - April 14th 2011

Where

LSI 4th floor CBR Fermentation Suite (Rm 4332) and Rm 4332

Attending:

  • Louise Creagh: Senior Instructor, Manager at Centre for BioThermodynamics, Laboratory of Molecular Biophysics
  • Raymond Pan: PhD Student, Department of Biochemistry and Molecular Biology(Bromme lab)
  • Swati Yewlakar: Postdoctoral Fellow, Department of Chemical and Biological Engineering
  • Tacky Loonchanta: PhD Student, Department of Chemistry (Burtnick lab)<-- Decision pending


Off-line Measurement

Day Group Run Temp DO pH Day OD Media Day D-1 D-2 D-3 ' Cell growth Phage Yield of Phage
-1 I 1 28.5 70.0 6.5 0 (OD= ) ( ml) 1 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
-1 I 2 28.5 70.0 10.7 0 (OD= ) ( ml) 1 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
-1 I 3 37.0 100.0 4.0 0 (OD= ) ( ml) 1 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
1 A 4 14.2 70.0 6.5 2 (OD= ) ( ml) 3 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
1 A 5 28.5 70.0 6.5 2 (OD= ) ( ml) 3 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
1 A 6 20.0 100.0 4.0 2 (OD= ) ( ml) 3 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
2 B 7 28.5 120.5 6.5 3 (OD= ) ( ml) 4 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
2 B 8 20.0 100.0 9.0 3 (OD= ) ( ml) 4 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
2 B 9 20.0 40.0 4.0 3 (OD= ) ( ml) 4 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
3 A 10 28.5 70.0 6.5 4 (OD= ) ( ml) 5 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
3 A 11 42.8 70.0 6.5 4 (OD= ) ( ml) 5 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
3 A 12 37.0 100.0 9.0 4 (OD= ) ( ml) 5 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
6 B 13 28.5 70.0 6.5 7 (OD= ) ( ml) 8 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
6 B 14 28.5 19.5 6.5 7 (OD= ) ( ml) 8 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
6 B 15 20.0 40.0 9.0 7 (OD= ) ( ml) 8 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
7 A 16 28.5 70.0 2.3 8 (OD= ) ( ml) 9 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
7 A 17 37.0 40.0 9.0 8 (OD= ) ( ml) 9 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
7 A 18 28.5 70.0 6.5 8 (OD= ) ( ml) 9 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
8 B 19 37.0 40.0 4.0 9 (OD= ) ( ml) 10 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)
8 B 20 28.5 70.0 6.5 9 (OD= ) ( ml) 10 (R1D1:data) (R1D2:data) (R1D3:data) (cell grams) (TU/total) (TU/grams)


Day -1 (April 4th, Mon)

Runs ' ' 1, 2, 3
07:30
08:00
09:00
09:30 SH: Assemble bioreactors
09:50 SH: Autoclve
10:00
10:30
11:10 SH: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation

Day 1 (April 5th, Tue) <-- WORKSHOP STARTS

Runs ' 1, 2, 3 4, 5, 6
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Ann: Sampling
09:30 Ann: Phage dilution Group A: Assemble bioreactors
09:50 Ann: OD of HD cells Group A: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group A: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # Introduction about our Facility and Services
 # Introduction about bioreactors
 # How to assemble bioreactors

Day 2 (April 6th, Wed)

Runs 1, 2, 3 4, 5, 6 7, 8, 9
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Ann: Colony check Group A: Sampling
09:30 Ann: Phage dilution Group B: Assemble bioreactors
09:50 Ann: OD of HD cells Group B: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # Fermentation Database and Websites
 # How to Install Bioreactors
 # How to Setup Bio-controllers



Day 3 (April 7th, Thu)

Runs 4, 5, 6 7, 8, 9 10, 11, 12
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group A: Colony check Group B: Sampling
09:30 Ann: Phage dilution Group A: Assemble bioreactors
09:50 Ann: OD of HD cells Group A: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group A: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 1. Design Experiments


Day 4 (April 8th, Fri)

Runs 7, 8, 9 10, 11, 12 '
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group B: Colony check Group A: Sampling
09:30 Ann: Phage dilution
09:50 Ann: OD of HD cells
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer
15:30
16:50
17:00 
NO PRESENTATION

Day 5 (April 9th, Sat)

Runs 10, 11, 12 ' '
07:30
08:00
09:00 SH: Colony check
09:30
09:50
10:00
10:30
11:10
15:30
16:50
17:00 
NO PRESENTATION


Day 6 (April 10th, Mon)

Runs ' ' 13, 14, 15
07:30
08:00
09:00
09:30 Group B: Assemble bioreactors
09:50 Group B: Autoclve
10:00
10:30
11:10 Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis of Responses (Part I): Fit Summary and Model Selection


Day 7 (April 11th, Tue)

Runs ' 13, 14, 15 16, 17, 18
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group B: Sampling
09:30 Ann: Phage dilution Group A: Assemble bioreactors
09:50 Ann: OD of HD cells Group A: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group A: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis of Responses (Part II): ANOVA, Diagnostics, and Model graphs

Day 8 (April 12th, Wed)

Runs 13, 14, 15 16, 17, 18 19, 20
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Ann: Colony check Group A: Sampling
09:30 Ann: Phage dilution Group B: Assemble bioreactors
09:50 Ann: OD of HD cells Group B: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 4. Optimization


Day 9 (April 13th, Thu)

Runs 16, 17, 18 19, 20 '
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group A: Colony check Group B: Sampling
09:30 Ann: Phage dilution
09:50 Ann: OD of HD cells
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer
15:30
16:50
17:00 
10:00 - 11:00 Presentation (Rm 4.303)
 # Case Studies: Publication

Helful links

  1. http://meta.wikimedia.org/wiki/Table
  2. http://excel2wiki.net/ <--- Use this (Copy and Paste Excel to wiki converter)!






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