User:Fermenter User/Notebook/DOE Workshop 20110405: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 28: Line 28:
==Off-line Measurement==
==Off-line Measurement==


{| class="wikitable sortable"
{| class="wikitable sortable" border="1"
|+Sortable table
|-
|-
! width="50pt"|Run !! width="50pt"|Std !! class="unsortable" | Group  || class="unsortable" | Day !! width="70pt"|Temp !! width="50pt"|DO !! width="50pt"|pH !! class="unsortable" | Day  !! width="50pt"|OD !! width="80pt"|Media !! class="unsortable" | Day  !! width="50pt"|Phage !! width="120pt"|Cell growth || width="80pt"|Growth || width="120pt"|Yield of Phage  
! width="50pt"|Run !! width="50pt"|Std !! class="unsortable" | Group  || class="unsortable" | Day !! width="70pt"|Temp !! width="50pt"|DO !! width="50pt"|pH !! class="unsortable" | Day  !! width="50pt"|OD !! width="80pt"|Media !! class="unsortable" | Day  !! width="50pt"|Phage !! width="120pt"|Cell growth || width="80pt"|Growth || width="120pt"|Yield of Phage  

Revision as of 14:31, 22 March 2011

WORK B1


1st LSI-CBR Design of Experiment (DOE) process Optimization Workshop

Teaching:


When

April 5th - April 14th 2011

Where

LSI 4th floor CBR Fermentation Suite (Rm 4332) and Rm 4332

Attending:

  • Louise Creagh: Senior Instructor, Manager at Centre for BioThermodynamics, Laboratory of Molecular Biophysics
  • Raymond Pan: PhD Student, Department of Biochemistry and Molecular Biology(Bromme lab)
  • Swati Yewlakar: Postdoctoral Fellow, Department of Chemical and Biological Engineering
  • Tacky Loonchanta: PhD Student, Department of Chemistry (Burtnick lab)<-- Decision pending



Off-line Measurement

Run Std Group Day Temp DO pH Day OD Media Day Phage Cell growth Growth Yield of Phage
1 15 i -1 28.5 70.0 6.5 0 (OD= ) ( ml) 1 (R1D1:data) (cell grams) (TU/total) (TU/grams)
2 14 i -1 28.5 70.0 10.7 0 (OD= ) ( ml) 1 (R1D1:data) (cell grams) (TU/total) (TU/grams)
3 4 i -1 37.0 100.0 4.0 0 (OD= ) ( ml) 1 (R1D1:data) (cell grams) (TU/total) (TU/grams)
4 9 a 1 14.2 70.0 6.5 2 (OD= ) ( ml) 3 (R1D1:data) (cell grams) (TU/total) (TU/grams)
5 17 a 1 28.5 70.0 6.5 2 (OD= ) ( ml) 3 (R1D1:data) (cell grams) (TU/total) (TU/grams)
6 3 a 1 20.0 100.0 4.0 2 (OD= ) ( ml) 3 (R1D1:data) (cell grams) (TU/total) (TU/grams)
7 12 b 2 28.5 120.5 6.5 3 (OD= ) ( ml) 4 (R1D1:data) (cell grams) (TU/total) (TU/grams)
8 7 b 2 20.0 100.0 9.0 3 (OD= ) ( ml) 4 (R1D1:data) (cell grams) (TU/total) (TU/grams)
9 1 b 2 20.0 40.0 4.0 3 (OD= ) ( ml) 4 (R1D1:data) (cell grams) (TU/total) (TU/grams)
10 19 a 3 28.5 70.0 6.5 4 (OD= ) ( ml) 5 (R1D1:data) (cell grams) (TU/total) (TU/grams)
11 10 a 3 42.8 70.0 6.5 4 (OD= ) ( ml) 5 (R1D1:data) (cell grams) (TU/total) (TU/grams)
12 8 a 3 37.0 100.0 9.0 4 (OD= ) ( ml) 5 (R1D1:data) (cell grams) (TU/total) (TU/grams)
13 16 b 6 28.5 70.0 6.5 7 (OD= ) ( ml) 8 (R1D1:data) (cell grams) (TU/total) (TU/grams)
14 11 b 6 28.5 19.5 6.5 7 (OD= ) ( ml) 8 (R1D1:data) (cell grams) (TU/total) (TU/grams)
15 5 b 6 20.0 40.0 9.0 7 (OD= ) ( ml) 8 (R1D1:data) (cell gams) (TU/total) (TU/grams)
16 13 a 7 28.5 70.0 2.3 8 (OD= ) ( ml) 9 (R1D1:data) (cell grams) (TU/total) (TU/grams)
17 6 a 7 37.0 40.0 9.0 8 (OD= ) ( ml) 9 (R1D1:data) (cell grams) (TU/total) (TU/grams)
18 18 a 7 28.5 70.0 6.5 8 (OD= ) ( ml) 9 (R1D1:data) (cell grams) (TU/total) (TU/grams)
19 2 b 8 37.0 40.0 4.0 9 (OD= ) ( ml) 10 (R1D1:data) (cell grams) (TU/total) (TU/grams)
20 20 b 8 28.5 70.0 6.5 9 (OD= ) ( ml) 10 (R1D1:data) (cell grams) (TU/total) (TU/grams)



Day -1 (April 4th, Mon)

Runs ' ' 1, 2, 3
07:30
08:00
09:00
09:30 SH: Assemble bioreactors
09:50 SH: Autoclve
10:00
10:30
11:10 SH: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation

Day 1 (April 5th, Tue) <-- WORKSHOP STARTS

Runs ' 1, 2, 3 4, 5, 6
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Ann: Sampling
09:30 Ann: Phage dilution Group A: Assemble bioreactors
09:50 Ann: OD of HD cells Group A: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group A: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # Introduction about our Facility and Services
 # Introduction about bioreactors
 # How to assemble bioreactors

Day 2 (April 6th, Wed)

Runs 1, 2, 3 4, 5, 6 7, 8, 9
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Ann: Colony check Group A: Sampling
09:30 Ann: Phage dilution Group B: Assemble bioreactors
09:50 Ann: OD of HD cells Group B: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # Fermentation Database and Websites
 # How to Install Bioreactors
 # How to Setup Bio-controllers



Day 3 (April 7th, Thu)

Runs 4, 5, 6 7, 8, 9 10, 11, 12
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group A: Colony check Group B: Sampling
09:30 Ann: Phage dilution Group A: Assemble bioreactors
09:50 Ann: OD of HD cells Group A: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group A: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 1. Design Experiments


Day 4 (April 8th, Fri)

Runs 7, 8, 9 10, 11, 12 '
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group B: Colony check Group A: Sampling
09:30 Ann: Phage dilution
09:50 Ann: OD of HD cells
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer
15:30
16:50
17:00 
NO PRESENTATION

Day 5 (April 9th, Sat)

Runs 10, 11, 12 ' '
07:30
08:00
09:00 SH: Colony check
09:30
09:50
10:00
10:30
11:10
15:30
16:50
17:00 
NO PRESENTATION


Day 6 (April 10th, Mon)

Runs ' ' 13, 14, 15
07:30
08:00
09:00
09:30 Group B: Assemble bioreactors
09:50 Group B: Autoclve
10:00
10:30
11:10 Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis of Responses (Part I): Fit Summary and Model Selection


Day 7 (April 11th, Tue)

Runs ' 13, 14, 15 16, 17, 18
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group B: Sampling
09:30 Ann: Phage dilution Group A: Assemble bioreactors
09:50 Ann: OD of HD cells Group A: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group A: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis of Responses (Part II): ANOVA, Diagnostics, and Model graphs

Day 8 (April 12th, Wed)

Runs 13, 14, 15 16, 17, 18 19, 20
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Ann: Colony check Group A: Sampling
09:30 Ann: Phage dilution Group B: Assemble bioreactors
09:50 Ann: OD of HD cells Group B: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 4. Optimization


Day 9 (April 13th, Thu)

Runs 16, 17, 18 19, 20 '
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group A: Colony check Group B: Sampling
09:30 Ann: Phage dilution
09:50 Ann: OD of HD cells
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer
15:30
16:50
17:00 
10:00 - 11:00 Presentation (Rm 4.303)
 # Case Studies: Publication


Day 10 (April 14th, Fri)

Runs 19, 20 ' '
07:30
08:00
09:00 Group B: Colony check
09:30
09:50
10:00
10:30
11:10
15:30
16:50
17:00 
9:30 - 12:00 DOE analysis with workshop data (Rm 4.307 Western Lantern)

Helful links

  1. http://meta.wikimedia.org/wiki/Table
  2. http://excel2wiki.net/ <--- Use this (Copy and Paste Excel to wiki converter)!






Recently Edited Notebook Pages


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Fermenter_User/Notebook/DOE_Workshop_20110405&oldid=500226"