User:Fermenter User/Notebook/DOE Workshop 20110405: Difference between revisions

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| 1||15||I||-1||28.5||70.0||6.5||0||(OD= )||( ml)||1||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)</font>
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| 2||14||i||-1||28.5||70.0||10.7||0||(OD= )||( ml)||1||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
| 2||14||I||-1||28.5||70.0||10.7||0||(OD= )||( ml)||1||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
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| 4||9||a||1||14.2||70.0||6.5||2||(OD= )||( ml)||3||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
| 4||9||A||1||14.2||70.0||6.5||2||(OD= )||( ml)||3||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
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|- style="color: red"
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| 6||3||A||1||20.0||100.0||4.0||2||(OD= )||( ml)||3||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
|- style="color: blue"
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| 7||12||b||2||28.5||120.5||6.5||3||(OD= )||( ml)||4||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
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| 8||7||b||2||20.0||100.0||9.0||3||(OD= )||( ml)||4||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
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| 12||8||a||3||37.0||100.0||9.0||4||(OD= )||( ml)||5||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
| 12||8||A||3||37.0||100.0||9.0||4||(OD= )||( ml)||5||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
|- style="color: blue"
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| 13||16||b||6||28.5||70.0||6.5||7||(OD= )||( ml)||8||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
| 13||16||B||6||28.5||70.0||6.5||7||(OD= )||( ml)||8||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
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| 14||11||b||6||28.5||19.5||6.5||7||(OD= )||( ml)||8||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
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| 15||5||b||6||20.0||40.0||9.0||7||(OD= )||( ml)||8||(R1D1:data)||(cell gams)||(TU/total)||(TU/grams)
| 15||5||B||6||20.0||40.0||9.0||7||(OD= )||( ml)||8||(R1D1:data)||(cell gams)||(TU/total)||(TU/grams)
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| 16||13||a||7||28.5||70.0||2.3||8||(OD= )||( ml)||9||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
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|- style="color: red"
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| 17||6||a||7||37.0||40.0||9.0||8||(OD= )||( ml)||9||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
| 17||6||A||7||37.0||40.0||9.0||8||(OD= )||( ml)||9||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
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|- style="color: red"
| 18||18||a||7||28.5||70.0||6.5||8||(OD= )||( ml)||9||(R1D1:data)||(cell grams)||(TU/total)||(TU/grams)
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Revision as of 14:52, 22 March 2011

WORK B1


1st LSI-CBR Design of Experiment (DOE) process Optimization Workshop

Teaching:


When

April 5th - April 14th 2011

Where

LSI 4th floor CBR Fermentation Suite (Rm 4332) and Rm 4332

Attending:

  • Louise Creagh: Senior Instructor, Manager at Centre for BioThermodynamics, Laboratory of Molecular Biophysics
  • Raymond Pan: PhD Student, Department of Biochemistry and Molecular Biology(Bromme lab)
  • Swati Yewlakar: Postdoctoral Fellow, Department of Chemical and Biological Engineering
  • Tacky Loonchanta: PhD Student, Department of Chemistry (Burtnick lab)<-- Decision pending



Off-line Measurement

Run Std Group Day Temp DO pH Day OD Media Day Phage Cell growth Growth Yield of Phage
1 15 I -1 28.5 70.0 6.5 0 (OD= ) ( ml) 1 (R1D1:data) (cell grams) (TU/total) (TU/grams)
2 14 I -1 28.5 70.0 10.7 0 (OD= ) ( ml) 1 (R1D1:data) (cell grams) (TU/total) (TU/grams)
3 4 I -1 37.0 100.0 4.0 0 (OD= ) ( ml) 1 (R1D1:data) (cell grams) (TU/total) (TU/grams)
4 9 A 1 14.2 70.0 6.5 2 (OD= ) ( ml) 3 (R1D1:data) (cell grams) (TU/total) (TU/grams)
5 17 A 1 28.5 70.0 6.5 2 (OD= ) ( ml) 3 (R1D1:data) (cell grams) (TU/total) (TU/grams)
6 3 A 1 20.0 100.0 4.0 2 (OD= ) ( ml) 3 (R1D1:data) (cell grams) (TU/total) (TU/grams)
7 12 B 2 28.5 120.5 6.5 3 (OD= ) ( ml) 4 (R1D1:data) (cell grams) (TU/total) (TU/grams)
8 7 B 2 20.0 100.0 9.0 3 (OD= ) ( ml) 4 (R1D1:data) (cell grams) (TU/total) (TU/grams)
9 1 B 2 20.0 40.0 4.0 3 (OD= ) ( ml) 4 (R1D1:data) (cell grams) (TU/total) (TU/grams)
10 19 A 3 28.5 70.0 6.5 4 (OD= ) ( ml) 5 (R1D1:data) (cell grams) (TU/total) (TU/grams)
11 10 A 3 42.8 70.0 6.5 4 (OD= ) ( ml) 5 (R1D1:data) (cell grams) (TU/total) (TU/grams)
12 8 A 3 37.0 100.0 9.0 4 (OD= ) ( ml) 5 (R1D1:data) (cell grams) (TU/total) (TU/grams)
13 16 B 6 28.5 70.0 6.5 7 (OD= ) ( ml) 8 (R1D1:data) (cell grams) (TU/total) (TU/grams)
14 11 B 6 28.5 19.5 6.5 7 (OD= ) ( ml) 8 (R1D1:data) (cell grams) (TU/total) (TU/grams)
15 5 B 6 20.0 40.0 9.0 7 (OD= ) ( ml) 8 (R1D1:data) (cell gams) (TU/total) (TU/grams)
16 13 A 7 28.5 70.0 2.3 8 (OD= ) ( ml) 9 (R1D1:data) (cell grams) (TU/total) (TU/grams)
17 6 A 7 37.0 40.0 9.0 8 (OD= ) ( ml) 9 (R1D1:data) (cell grams) (TU/total) (TU/grams)
18 18 A 7 28.5 70.0 6.5 8 (OD= ) ( ml) 9 (R1D1:data) (cell grams) (TU/total) (TU/grams)
19 2 B 8 37.0 40.0 4.0 9 (OD= ) ( ml) 10 (R1D1:data) (cell grams) (TU/total) (TU/grams)
20 20 B 8 28.5 70.0 6.5 9 (OD= ) ( ml) 10 (R1D1:data) (cell grams) (TU/total) (TU/grams)



Day -1 (April 4th, Mon)

Runs ' ' 1, 2, 3
07:30
08:00
09:00
09:30 SH: Assemble bioreactors
09:50 SH: Autoclve
10:00
10:30
11:10 SH: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation

Day 1 (April 5th, Tue) <-- WORKSHOP STARTS

Runs ' 1, 2, 3 4, 5, 6
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Ann: Sampling
09:30 Ann: Phage dilution Group A: Assemble bioreactors
09:50 Ann: OD of HD cells Group A: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group A: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # Introduction about our Facility and Services
 # Introduction about bioreactors
 # How to assemble bioreactors

Day 2 (April 6th, Wed)

Runs 1, 2, 3 4, 5, 6 7, 8, 9
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Ann: Colony check Group A: Sampling
09:30 Ann: Phage dilution Group B: Assemble bioreactors
09:50 Ann: OD of HD cells Group B: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # Fermentation Database and Websites
 # How to Install Bioreactors
 # How to Setup Bio-controllers



Day 3 (April 7th, Thu)

Runs 4, 5, 6 7, 8, 9 10, 11, 12
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group A: Colony check Group B: Sampling
09:30 Ann: Phage dilution Group A: Assemble bioreactors
09:50 Ann: OD of HD cells Group A: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group A: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 1. Design Experiments


Day 4 (April 8th, Fri)

Runs 7, 8, 9 10, 11, 12 '
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group B: Colony check Group A: Sampling
09:30 Ann: Phage dilution
09:50 Ann: OD of HD cells
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer
15:30
16:50
17:00 
NO PRESENTATION

Day 5 (April 9th, Sat)

Runs 10, 11, 12 ' '
07:30
08:00
09:00 SH: Colony check
09:30
09:50
10:00
10:30
11:10
15:30
16:50
17:00 
NO PRESENTATION


Day 6 (April 10th, Mon)

Runs ' ' 13, 14, 15
07:30
08:00
09:00
09:30 Group B: Assemble bioreactors
09:50 Group B: Autoclve
10:00
10:30
11:10 Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis of Responses (Part I): Fit Summary and Model Selection


Day 7 (April 11th, Tue)

Runs ' 13, 14, 15 16, 17, 18
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group B: Sampling
09:30 Ann: Phage dilution Group A: Assemble bioreactors
09:50 Ann: OD of HD cells Group A: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group A: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 3. Analysis of Responses (Part II): ANOVA, Diagnostics, and Model graphs

Day 8 (April 12th, Wed)

Runs 13, 14, 15 16, 17, 18 19, 20
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Ann: Colony check Group A: Sampling
09:30 Ann: Phage dilution Group B: Assemble bioreactors
09:50 Ann: OD of HD cells Group B: Autoclve
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer Group B: Install bioreactors
15:30 SH: Thaw stock
16:50 SH: Pre-culture for HD K19 cells
17:00 SH: Inoculation 
10:00 - 11:00 Presentation (Rm 4.303)
 # DOE Step 4. Optimization


Day 9 (April 13th, Thu)

Runs 16, 17, 18 19, 20 '
07:30 SH: Prepare hight density K19 cells
08:00 SH: Prepare dilution tubes
09:00 Group A: Colony check Group B: Sampling
09:30 Ann: Phage dilution
09:50 Ann: OD of HD cells
10:00 Ann: Dry plates
10:30 Ann: Infect K19 cells
11:10 Ann: Spot titer
15:30
16:50
17:00 
10:00 - 11:00 Presentation (Rm 4.303)
 # Case Studies: Publication


Day 10 (April 14th, Fri)

Runs 19, 20 ' '
07:30
08:00
09:00 Group B: Colony check
09:30
09:50
10:00
10:30
11:10
15:30
16:50
17:00 
9:30 - 12:00 DOE analysis with workshop data (Rm 4.307 Western Lantern)

Helful links

  1. http://meta.wikimedia.org/wiki/Table
  2. http://excel2wiki.net/ <--- Use this (Copy and Paste Excel to wiki converter)!






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