WORK B1
1st LSI-CBR Design of Experiment (DOE) process Optimization Workshop
Teaching:
When
April 5th - April 14th 2011
Where
LSI 4th floor CBR Fermentation Suite (Rm 4332) and Rm 4332
Attending:
- Louise Creagh: Senior Instructor, Manager at Centre for BioThermodynamics, Laboratory of Molecular Biophysics
- Raymond Pan: PhD Student, Department of Biochemistry and Molecular Biology(Bromme lab)
- Swati Yewlakar: Postdoctoral Fellow, Department of Chemical and Biological Engineering
- Tacky Loonchanta: PhD Student, Department of Chemistry (Burtnick lab)<-- Decision pending
Group A:
Group B:
Off-line Measurement
NOTE: This is a Sortable table. Click triangle symbol next to some headings to sort.
Run |
Std |
Group |
Day |
Temp |
DO |
pH |
Day |
OD |
Media |
Day |
Phage |
Cell growth |
Growth |
Yield of Phage
|
1 |
15 |
I |
-1 |
28.5 |
70.0 |
6.5 |
0 |
(OD= ) |
( ml) |
1 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
2 |
14 |
I |
-1 |
28.5 |
70.0 |
10.7 |
0 |
(OD= ) |
( ml) |
1 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
3 |
4 |
I |
-1 |
37.0 |
100.0 |
4.0 |
0 |
(OD= ) |
( ml) |
1 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
4 |
9 |
A |
1 |
14.2 |
70.0 |
6.5 |
2 |
(OD= ) |
( ml) |
3 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
5 |
17 |
A |
1 |
28.5 |
70.0 |
6.5 |
2 |
(OD= ) |
( ml) |
3 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
6 |
3 |
A |
1 |
20.0 |
100.0 |
4.0 |
2 |
(OD= ) |
( ml) |
3 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
7 |
12 |
B |
2 |
28.5 |
120.5 |
6.5 |
3 |
(OD= ) |
( ml) |
4 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
8 |
7 |
B |
2 |
20.0 |
100.0 |
9.0 |
3 |
(OD= ) |
( ml) |
4 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
9 |
1 |
B |
2 |
20.0 |
40.0 |
4.0 |
3 |
(OD= ) |
( ml) |
4 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
10 |
19 |
A |
3 |
28.5 |
70.0 |
6.5 |
4 |
(OD= ) |
( ml) |
5 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
11 |
10 |
A |
3 |
42.8 |
70.0 |
6.5 |
4 |
(OD= ) |
( ml) |
5 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
12 |
8 |
A |
3 |
37.0 |
100.0 |
9.0 |
4 |
(OD= ) |
( ml) |
5 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
13 |
16 |
B |
6 |
28.5 |
70.0 |
6.5 |
7 |
(OD= ) |
( ml) |
8 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
14 |
11 |
B |
6 |
28.5 |
19.5 |
6.5 |
7 |
(OD= ) |
( ml) |
8 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
15 |
5 |
B |
6 |
20.0 |
40.0 |
9.0 |
7 |
(OD= ) |
( ml) |
8 |
(R1D1:data) |
(cell gams) |
(TU/total) |
(TU/grams)
|
16 |
13 |
A |
7 |
28.5 |
70.0 |
2.3 |
8 |
(OD= ) |
( ml) |
9 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
17 |
6 |
A |
7 |
37.0 |
40.0 |
9.0 |
8 |
(OD= ) |
( ml) |
9 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
18 |
18 |
A |
7 |
28.5 |
70.0 |
6.5 |
8 |
(OD= ) |
( ml) |
9 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
19 |
2 |
B |
8 |
37.0 |
40.0 |
4.0 |
9 |
(OD= ) |
( ml) |
10 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
20 |
20 |
B |
8 |
28.5 |
70.0 |
6.5 |
9 |
(OD= ) |
( ml) |
10 |
(R1D1:data) |
(cell grams) |
(TU/total) |
(TU/grams)
|
|
Day -1 (April 4th, Mon)
Runs
|
'
|
'
|
1, 2, 3
|
07:30 |
|
|
|
08:00 |
|
|
|
09:00 |
|
|
|
09:30 |
|
|
SH: Assemble bioreactors
|
09:50 |
|
|
SH: Autoclve
|
10:00 |
|
|
|
10:30 |
|
|
|
11:10 |
|
|
SH: Install bioreactors
|
15:30 |
|
|
SH: Thaw stock
|
16:50 |
|
|
SH: Pre-culture for HD K19 cells
|
17:00 |
|
|
SH: Inoculation
|
|
Day 1 (April 5th, Tue) <-- WORKSHOP STARTS
Runs
|
'
|
1, 2, 3
|
4, 5, 6
|
07:30 |
|
SH: Prepare hight density K19 cells |
|
08:00 |
|
SH: Prepare dilution tubes |
|
09:00 |
|
Ann: Sampling |
|
09:30 |
|
Ann: Phage dilution |
Group A: Assemble bioreactors
|
09:50 |
|
Ann: OD of HD cells |
Group A: Autoclve
|
10:00 |
|
Ann: Dry plates |
|
10:30 |
|
Ann: Infect K19 cells |
|
11:10 |
|
Ann: Spot titer |
Group A: Install bioreactors
|
15:30 |
|
|
SH: Thaw stock
|
16:50 |
|
|
SH: Pre-culture for HD K19 cells
|
17:00 |
|
|
SH: Inoculation
|
|
10:00 - 11:00 Presentation (Rm 4.303)
# Introduction about our Facility and Services
# Introduction about bioreactors
# How to assemble bioreactors
Day 2 (April 6th, Wed)
Runs
|
1, 2, 3
|
4, 5, 6
|
7, 8, 9
|
07:30 |
|
SH: Prepare hight density K19 cells |
|
08:00 |
|
SH: Prepare dilution tubes |
|
09:00 |
Ann: Colony check |
Group A: Sampling |
|
09:30 |
|
Ann: Phage dilution |
Group B: Assemble bioreactors
|
09:50 |
|
Ann: OD of HD cells |
Group B: Autoclve
|
10:00 |
|
Ann: Dry plates |
|
10:30 |
|
Ann: Infect K19 cells |
|
11:10 |
|
Ann: Spot titer |
Group B: Install bioreactors
|
15:30 |
|
|
SH: Thaw stock
|
16:50 |
|
|
SH: Pre-culture for HD K19 cells
|
17:00 |
|
|
SH: Inoculation
|
|
10:00 - 11:00 Presentation (Rm 4.303)
# Fermentation Database and Websites
# How to Install Bioreactors
# How to Setup Bio-controllers
Day 3 (April 7th, Thu)
Runs
|
4, 5, 6
|
7, 8, 9
|
10, 11, 12
|
07:30 |
|
SH: Prepare hight density K19 cells |
|
08:00 |
|
SH: Prepare dilution tubes |
|
09:00 |
Group A: Colony check |
Group B: Sampling |
|
09:30 |
|
Ann: Phage dilution |
Group A: Assemble bioreactors
|
09:50 |
|
Ann: OD of HD cells |
Group A: Autoclve
|
10:00 |
|
Ann: Dry plates |
|
10:30 |
|
Ann: Infect K19 cells |
|
11:10 |
|
Ann: Spot titer |
Group A: Install bioreactors
|
15:30 |
|
|
SH: Thaw stock
|
16:50 |
|
|
SH: Pre-culture for HD K19 cells
|
17:00 |
|
|
SH: Inoculation
|
|
10:00 - 11:00 Presentation (Rm 4.303)
# DOE Step 1. Design Experiments
Day 4 (April 8th, Fri)
Runs
|
7, 8, 9
|
10, 11, 12
|
'
|
07:30 |
|
SH: Prepare hight density K19 cells |
|
08:00 |
|
SH: Prepare dilution tubes |
|
09:00 |
Group B: Colony check |
Group A: Sampling |
|
09:30 |
|
Ann: Phage dilution |
|
09:50 |
|
Ann: OD of HD cells |
|
10:00 |
|
Ann: Dry plates |
|
10:30 |
|
Ann: Infect K19 cells |
|
11:10 |
|
Ann: Spot titer |
|
15:30 |
|
|
|
16:50 |
|
|
|
17:00 |
|
|
|
|
NO PRESENTATION
Day 5 (April 9th, Sat)
Runs
|
10, 11, 12
|
'
|
'
|
07:30 |
|
|
|
08:00 |
|
|
|
09:00 |
SH: Colony check |
|
|
09:30 |
|
|
|
09:50 |
|
|
|
10:00 |
|
|
|
10:30 |
|
|
|
11:10 |
|
|
|
15:30 |
|
|
|
16:50 |
|
|
|
17:00 |
|
|
|
|
NO PRESENTATION
Day 6 (April 10th, Mon)
Runs
|
'
|
'
|
13, 14, 15
|
07:30 |
|
|
|
08:00 |
|
|
|
09:00 |
|
|
|
09:30 |
|
|
Group B: Assemble bioreactors
|
09:50 |
|
|
Group B: Autoclve
|
10:00 |
|
|
|
10:30 |
|
|
|
11:10 |
|
|
Group B: Install bioreactors
|
15:30 |
|
|
SH: Thaw stock
|
16:50 |
|
|
SH: Pre-culture for HD K19 cells
|
17:00 |
|
|
SH: Inoculation
|
|
10:00 - 11:00 Presentation (Rm 4.303)
# DOE Step 3. Analysis of Responses (Part I): Fit Summary and Model Selection
Day 7 (April 11th, Tue)
Runs
|
'
|
13, 14, 15
|
16, 17, 18
|
07:30 |
|
SH: Prepare hight density K19 cells |
|
08:00 |
|
SH: Prepare dilution tubes |
|
09:00 |
|
Group B: Sampling |
|
09:30 |
|
Ann: Phage dilution |
Group A: Assemble bioreactors
|
09:50 |
|
Ann: OD of HD cells |
Group A: Autoclve
|
10:00 |
|
Ann: Dry plates |
|
10:30 |
|
Ann: Infect K19 cells |
|
11:10 |
|
Ann: Spot titer |
Group A: Install bioreactors
|
15:30 |
|
|
SH: Thaw stock
|
16:50 |
|
|
SH: Pre-culture for HD K19 cells
|
17:00 |
|
|
SH: Inoculation
|
|
10:00 - 11:00 Presentation (Rm 4.303)
# DOE Step 3. Analysis of Responses (Part II): ANOVA, Diagnostics, and Model graphs
Day 8 (April 12th, Wed)
Runs
|
13, 14, 15
|
16, 17, 18
|
19, 20
|
07:30 |
|
SH: Prepare hight density K19 cells |
|
08:00 |
|
SH: Prepare dilution tubes |
|
09:00 |
Ann: Colony check |
Group A: Sampling |
|
09:30 |
|
Ann: Phage dilution |
Group B: Assemble bioreactors
|
09:50 |
|
Ann: OD of HD cells |
Group B: Autoclve
|
10:00 |
|
Ann: Dry plates |
|
10:30 |
|
Ann: Infect K19 cells |
|
11:10 |
|
Ann: Spot titer |
Group B: Install bioreactors
|
15:30 |
|
|
SH: Thaw stock
|
16:50 |
|
|
SH: Pre-culture for HD K19 cells
|
17:00 |
|
|
SH: Inoculation
|
|
10:00 - 11:00 Presentation (Rm 4.303)
# DOE Step 4. Optimization
Day 9 (April 13th, Thu)
Runs
|
16, 17, 18
|
19, 20
|
'
|
07:30 |
|
SH: Prepare hight density K19 cells |
|
08:00 |
|
SH: Prepare dilution tubes |
|
09:00 |
Group A: Colony check |
Group B: Sampling |
|
09:30 |
|
Ann: Phage dilution |
|
09:50 |
|
Ann: OD of HD cells |
|
10:00 |
|
Ann: Dry plates |
|
10:30 |
|
Ann: Infect K19 cells |
|
11:10 |
|
Ann: Spot titer |
|
15:30 |
|
|
|
16:50 |
|
|
|
17:00 |
|
|
|
|
10:00 - 11:00 Presentation (Rm 4.303)
# Case Studies: Publication
Day 10 (April 14th, Fri)
Runs
|
19, 20
|
'
|
'
|
07:30 |
|
|
|
08:00 |
|
|
|
09:00 |
Group B: Colony check |
|
|
09:30 |
|
|
|
09:50 |
|
|
|
10:00 |
|
|
|
10:30 |
|
|
|
11:10 |
|
|
|
15:30 |
|
|
|
16:50 |
|
|
|
17:00 |
|
|
|
|
9:30 - 12:00 DOE analysis with workshop data (Rm 4.307 Western Lantern)
Helful links
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