User:Fermenter User/Notebook/DOE Workshop 20110405: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''Runs''' | | align="center" style="background:#f0f0f0;"|'''Runs''' | ||
| align="center" style="background:#f0f0f0;"|'''4, 5, 6''' | |||
| align="center" style="background:#f0f0f0;"|'''7, 8, 9''' | | align="center" style="background:#f0f0f0;"|'''7, 8, 9''' | ||
| align="center" style="background:#f0f0f0;"|'''10, 11, 12''' | | align="center" style="background:#f0f0f0;"|'''10, 11, 12''' | ||
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| | | pictures||[[Image:colonys_456.jpg|400px|thumb|center|run 4,5,6 (left to right) ]] || [[Image:fermentation_789.jpg|400px|thumb|center|run 7,8,9 (left to right) ]] || | ||
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| 08:00||||<font color=gray> SH: | | 08:00||<font color=#734A12> Ann: Colony counting </font>|| <font color=gray> SH: Sampling </font>|| | ||
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| 09:00||<font color= | | 09:00||||<font color=#734A12> Ann: Prepare high density K91 cells <br> <font style:background-color="yellow"><--didn't grow very well due to power outage </font></font>||<font color=red> Group A: Assemble bioreactors </font> | ||
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| 09: | | 09:50||||||<font color=red> Group A: Autoclave </font> | ||
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| | | 10:00||Presentation||Presentation|| Presentation | ||
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| | | 11:00||||<font color=#734A12> </font>||<font color=red> Group A: Install bioreactors</font> | ||
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| | | 12:00||||<font color=#734A12> </font>|| | ||
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| | | 15:30||||||<font color=gray> SH: Thaw stock | ||
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| | | 16:50||||||<font color=gray> SH: Pre-culture for HD K91 cells | ||
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| | | 17:00||||||<font color=gray> SH: Inoculation | ||
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|} | |} | ||
NO PRESENTATION | NO PRESENTATION | ||
Revision as of 10:49, 8 April 2011
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WORK B1
Teaching:
Where: LSI 4th floor CBR Fermentation Suite (Rm 4332) and Rm 4332 Attending:
Group A:(After you log in with your personal password, click the "EDIT" button on the right side of GROUP A and list your name below) Chris Tam
Group B:(After you log in with your personal password, click the "EDIT" button on the right side of GROUP B and list your name below) MARY TANG
Off-line Measurement
Day -1 (April 4th, Mon)
Day 1 (April 5th, Tue) <-- WORKSHOP STARTS
10:00 - 11:00 Presentation (Rm 4.303) # Introduction about our Facility and Services # Introduction about bioreactors # How to assemble bioreactors Power Outage started around 8pm and lasted approximately 2.5 hrs. Bio-controllers and computer were connected to UPS (undisrupted power suppliers). Unfortunately, data has been lost due to power outage. Run #4 losted power during fermentation. End point looked ok. Run #5 was not affected by power. However, pH control was not done properly. Run #6 was not affected by power. End point looked ok. => Run #5 will be repeated at last as additional runs. Day 2 (April 6th, Wed)
10:00 - 11:00 Presentation (Rm 4.303) # Fermentation Database and Websites # How to Install Bioreactors # How to Setup Bio-controllers Another Power Outage started around 6:45pm and lasted approximately 1.5 hrs. Bio-controllers and computer were connected to UPS (undisrupted power suppliers). Unfortunately, data has been lost due to power outage. Run #7 losted power during fermentation. End point looked ok. Run #8 was not affected by power. End point looked ok. Run #9 was not affected by power. End point looked ok. => Run #7 will be repeated at last as additional runs. Day 3 (April 7th, Thu)
10:00 - 11:00 Presentation (Rm 4.303) # DOE Step 1. Design Experiments Due to unnotified power outage, two of emergency power lines were introduced into fermentation suite. One was connected to a computer and one was connected to incubator. Also, each fermenter is now connected to batteries to provide over 500 min capacity. Day 4 (April 8th, Fri)
NO PRESENTATION Day 5 (April 9th, Sat)
NO PRESENTATION
Day 6 (April 10th, Mon)
10:00 - 11:00 Presentation (Rm 4.303) # DOE Step 3. Analysis of Responses (Part I): Fit Summary and Model Selection
Day 7 (April 11th, Tue)
10:00 - 11:00 Presentation (Rm 4.303) # DOE Step 3. Analysis of Responses (Part II): ANOVA, Diagnostics, and Model graphs Day 8 (April 12th, Wed)
10:00 - 11:00 Presentation (Rm 4.303) # DOE Step 4. Optimization Day 9 (April 13th, Thu)
10:00 - 11:00 Presentation (Rm 4.303) # Case Studies: Publication Day 10 (April 14th, Fri)
9:30 - 12:00 DOE analysis with workshop data (Rm 4.307 Western Lantern) Helful links
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