NOTE: This is a Sortable table. Click triangle symbol next to some headings to sort.
Run
Std
Group
Day
Temp
DO
pH
Day
OD
Media (ml)
Day
Response 1
Response 2
Response 3
1
15
I
-1
NA
NA
NA
0
3.500
1080
1
11.064
6.20E+11
5.60E+10
2
14
I
-1
NA
NA
NA
0
0.032
840
1
0.079
2.78E+08
3.54E+09
3
4
I
-1
NA
NA
NA
0
0.263
650
1
0.500
2.60E+09
5.20E+09
6
3
A
1
NA
NA
NA
2
0.055
670
3
0.108
4.46E+09
4.14E+10
8
7
B
2
NA
NA
NA
3
0.146
660
4
0.282
7.79E+11
2.76E+12
9
1
B
2
NA
NA
NA
3
0.058
690
4
0.117
5.24E+09
4.47E+10
10
19
A
3
NA
NA
NA
4
6.149
640
5
11.519
(R10)
(R10)
12
8
A
3
NA
NA
NA
4
0.017
650
5
0.032
4.15E+11
1.28E+13
11
10
I
4
NA
NA
NA
5
6.345
650
6
(R11)
(R11)
(R11)
13
16
I
4
NA
NA
NA
5
7.143
650
6
(R13)
(R13)
(R13)
14
11
I
4
NA
NA
NA
5
7.830
650
6
(R14)
(R14)
(R14)
15
5
B
6
NA
NA
NA
7
(OD= )
( ml)
8
(R15)
(R15)
(R15)
16
13
B
6
NA
NA
NA
7
(OD= )
( ml)
8
(R16)
(R16)
(R16)
17
6
B
6
NA
NA
NA
7
(OD= )
( ml)
8
(R17)
(R17)
(R17)
18
18
A
7
NA
NA
NA
8
(OD= )
( ml)
9
(R18)
(R18)
(R18)
19
2
A
7
NA
NA
NA
8
(OD= )
( ml)
9
(R19)
(R19)
(R19)
20
20
A
7
NA
NA
NA
8
(OD= )
( ml)
9
(R20)
(R20)
(R20)
4
9
B
8
NA
NA
NA
9
(OD= )
( ml)
10
(R4)
(R4)
(R4)
5
17
B
8
NA
NA
NA
9
(OD=)
( ml)
10
(R5)
(R5)
(R5)
7
12
B
8
NA
NA
NA
9
(OD= )
( ml)
10
(R7)
(R7)
(R7)
Day -1 (April 4th, Mon)
Runs
'
'
1, 2, 3
07:30
08:00
09:00
09:30
SH: Assemble bioreactors
09:50
SH: Autoclve
10:00
10:30
11:10
SH: Install bioreactors
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K19 cells
17:00
SH: Inoculation
Day 1 (April 5th, Tue) <-- WORKSHOP STARTS
Runs
'
1, 2, 3
4, 5, 6
pictures
run 1, 2, 3 (left to right)
08:30
SH: Sampling
09:30
Ann: Prepare high density K91 cells
Group A: Assemble bioreactors
09:50
Group A: Autoclave
10:40
Presentation
Presentation
12:00
Ann: Phage infection
Group A: Install bioreactors
13:00
Ann: Spot titer
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K19 cells
17:00
SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
# Introduction about our Facility and Services
# Introduction about bioreactors
# How to assemble bioreactors
Power Outage started around 8pm and lasted approximately 2.5 hrs.
Bio-controllers and computer were connected to UPS (undisrupted power suppliers).
Unfortunately, data has been lost due to power outage.
Run #4 losted power during fermentation. End point looked ok.
Run #5 was not affected by power. However, pH control was not done properly.
Run #6 was not affected by power. End point looked ok.
=> Run #5 will be repeated at last as additional runs.
Day 2 (April 6th, Wed)
Runs
1, 2, 3
4, 5, 6
7, 8, 9
pictures
run 1,2,3 (left to right)
run 4,5,6 (left to right)
08:00
Ann: Colony counting
SH: Sampling
09:00
Ann: Prepare high density K91 cells
Group B: Assemble bioreactors
09:50
Group B: Autoclave
10:00
Presentation
Presentation
Presentation
11:00
Ann: Phage infection
Group B: Install bioreactors
12:00
Ann: Spot titer
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K91 cells
17:00
SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
# Fermentation Database and Websites
# How to Install Bioreactors
# How to Setup Bio-controllers
Another Power Outage started around 6:45pm and lasted approximately 1.5 hrs.
Bio-controllers and computer were connected to UPS (undisrupted power suppliers).
Unfortunately, data has been lost due to power outage.
Run #7 losted power during fermentation. End point looked ok.
Run #8 was not affected by power. End point looked ok.
Run #9 was not affected by power. End point looked ok.
=> Run #7 will be repeated at last as additional runs.
Day 3 (April 7th, Thu)
Runs
4, 5, 6
7, 8, 9
10, 11, 12
pictures
run 4,5,6 (left to right)
run 7,8,9 (left to right)
08:00
Ann: Colony counting
SH: Sampling
09:00
Ann: Prepare high density K91 cells <--didn't grow very well due to power outage
Due to unnotified power outage, two of emergency power lines were introduced into fermentation suite.
One was connected to a computer and one was connected to incubator.
Also, each fermenter is now connected to batteries to provide over 500 min capacity.
Day 4 (April 8th, Fri)
Runs
7, 8, 9
10, 11, 12
11, 13, 14
pictures
colony 7,8,9 (left to right)
run 10,11,12 (left to right)
08:00
SH: Sampling
09:00
Ann: Prepare high density K91 cells
SH: Assemble bioreactors
09:50
SH: Autoclave
11:00
Ann: Phage Infection
SH: Install bioreactors
12:00
Ann: Spot titering
15:30
Thaw stock
16:50
17:00
Inoculation
NO PRESENTATION
Day 5 (April 9th, Sat)
There is a scheduled power outage on Saturday morning between 8:00 AM and 10:00 AM
=> Power outage began at 8:01 and resumed at 10:00.
No problem occured during this power outage
Runs
6, 8, 9, 10, 12
11, 13, 14
'
pictures
run 10, 12, (left to right)
run 11,13,14 (left to right)
08:00
SH: Colony counting
SH: Sampling
NO PRESENTATION
Day 6 (April 10th, Mon)
Runs
'
'
15, 16, 17
07:30
08:00
09:00
09:30
Group B: Assemble bioreactors
09:50
Group B: Autoclve
10:00
10:30
11:10
Group B: Install bioreactors
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K19 cells
17:00
SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
# DOE Step 3. Analysis of Responses (Part I): Fit Summary and Model Selection
Day 7 (April 11th, Tue)
Runs
'
15, 16, 17
18, 19, 20
07:30
SH: Prepare hight density K19 cells
08:00
SH: Prepare dilution tubes
09:00
SH: Sampling
09:30
Ann: Phage dilution
Group A: Assemble bioreactors
09:50
Ann: OD of HD cells
Group A: Autoclve
10:00
Ann: Dry plates
10:30
Ann: Infect K19 cells
11:10
Ann: Spot titer
Group A: Install bioreactors
15:30
SH: Thaw stock
16:50
SH: Pre-culture for HD K19 cells
17:00
SH: Inoculation
10:00 - 11:00 Presentation (Rm 4.303)
# DOE Step 3. Analysis of Responses (Part II): ANOVA, Diagnostics, and Model graphs
