User:Floriane Briere/Notebook/CHEM-496/2011/09/14: Difference between revisions
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[[Image:14sept - Absorbance = f(wavelength).png]] | [[Image:14sept - Absorbance = f(wavelength).png]] | ||
* Curve of the as a function of the Wavelength (nm) | * Curve of the molar absorptivity (ε) as a function of the Wavelength (nm) | ||
==Notes== | ==Notes== |
Revision as of 05:35, 20 September 2011
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ObjectiveToday's experiment is to test the Bradford Assay technique which is going to allow us to determine the unknown proteins’ concentration of a solution. We are going to use 6 standards solutions of BSA whose concentrations are known. Then, thanks to a spectrophotometer, we’ll be able to determine the concentration of the MBP solution. Protocol
Results
Red lines represent the Absorbance at 595nm of the 1/1000 diluted MPB solution. This curve allow us to determine the concentration of the 1/1000 diluted MPB solution. To draw this curve, we add to take into account that the Bradford Assay was made with 5/6 diluted solutions (because we added 1ml of solution to 200µl of Bradford reagent). According to this curve, the 1/1000 diluted MPB solution has a concentration of 0.0276nM.
NotesThis area is for any observations or conclusions that you would like to note.
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