User:Floriane Briere/Notebook/CHEM-496/2011/09/27: Difference between revisions

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* Gold NPs synthesis by using Tris buffer. During the experiment, the solution is left at 80°C during the entire experiment
* Gold NPs synthesis by using Tris buffer. During the experiment, the solution is left at 80°C during the entire experiment


==Description==
==Protocol==
# Add experimental record here. Include what, how, and why...
 
* Gold NPs synthesis
# Add 0.97ml of BSA (15.4µM) + 0.58ml HAuCl4 (4.3mM) + 8.45 buffer (50mM Tris;pH 7.5) to get a 10ml solution ([BSA] = 1.5µM ; [HAuCl4] = 0.25mM)
# Take a spectrum at T0
# Place the solution in a hoven at 80°C


==Data==
==Data==

Revision as of 10:05, 27 September 2011

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Objective

  • Digest unmethyled DNA to isolate methyled DNA.
  • Gold NPs synthesis by using Tris buffer. During the experiment, the solution is left at 80°C during the entire experiment

Protocol

  • Gold NPs synthesis
  1. Add 0.97ml of BSA (15.4µM) + 0.58ml HAuCl4 (4.3mM) + 8.45 buffer (50mM Tris;pH 7.5) to get a 10ml solution ([BSA] = 1.5µM ; [HAuCl4] = 0.25mM)
  2. Take a spectrum at T0
  3. Place the solution in a hoven at 80°C

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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