User:Floriane Briere/Notebook/CHEM-496/2011/09/27: Difference between revisions
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==Results: DNA's transfer into cells== | ==Results: DNA's transfer into cells== | ||
The first step of the experiment was to add Dpnl enzyme to the PCR solution. This step allowed us to digest unmethylated DNA; we selected methylated DNA for the transfer. | The first step of the experiment was to add Dpnl enzyme to the PCR solution. This step allowed us to digest unmethylated DNA; we selected methylated DNA for the transfer. |
Revision as of 11:50, 19 October 2011
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ObjectiveWe are going to conduct two different experiments:
Protocol
Results: Gold NPs synthesis
At the end of the experiment, we obtained an incolore solution. We can observe that there is no significant increase of the Absorbance at 550nm during the experiment. Compare to previous results, we can say that Gold NPs synthesis is not efficient in Tris buffer. This result is quite similar to the one we obtained when we used acetate as a buffer (on the 7th of september). We obtained better results when we used water as a buffer (on the 31st of august). The next experiment is to make the same protocol, using water as a buffer, but with a stable temperature. Results: DNA's transfer into cellsThe first step of the experiment was to add Dpnl enzyme to the PCR solution. This step allowed us to digest unmethylated DNA; we selected methylated DNA for the transfer. Then, we transferred the DNA into novablue cells; cells which integrate our DNA molecules are then selected by adding Ampicillin to the agar/LB plate. In fact, the DNA molecule contains an antibiotics resistant gene. The cells were then placed into a favorable environment to allow them to grow up. After 24hours of incubation, we observed no cells on our plate.
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