User:Floriane Briere/Notebook/CHEM-496/2011/09/28: Difference between revisions

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==Objective==
==Objective==
We used the same protocol as on the 27th of September, except that we used water as a bufer instead of Tris (whose pH was equal to 7.5). According to our previous experiments, we have already observed that the Gold NPs was more efficient in water, compare to Tris buffer. So, we should observ better results than the one we had on the 27th of September.
We used the same protocol as on the 27th of September, except that we used water as a bufer instead of Tris (whose pH was equal to 7.5). According to our previous experiments, we have already observed that the Gold NPs was more efficient in water, compare to Tris or acetate buffer. So, we should observ better results than the one we had on the 27th of September.


==Description==
==Description==

Revision as of 19:04, 3 October 2011

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Objective

We used the same protocol as on the 27th of September, except that we used water as a bufer instead of Tris (whose pH was equal to 7.5). According to our previous experiments, we have already observed that the Gold NPs was more efficient in water, compare to Tris or acetate buffer. So, we should observ better results than the one we had on the 27th of September.

Description

  • Gold NPs synthesis:
  1. Add 0.97ml of BSA (15.4µM) + 0.58ml HAuCl4 (4.3mM) + 8.45 water
  2. We obtain a 10ml solution ([BSA] = 1.5µM ; [HAuCl4] = 0.25mM)
  3. Take a spectrum at T0
  4. Place the solution in the oven at 80°C during 2 hours
  5. Take spectrum every 30 minutes

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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