User:Floriane Briere/Notebook/CHEM-496/2011/10/19: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2011/10/19 Entry for User:Floriane_Briere/Notebook/CHEM-496)
 
No edit summary
(4 intermediate revisions by one other user not shown)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #EEE"|[[Image:BDLlogo_notext_ir.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_1r.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
Line 11: Line 11:


==Objective==
==Objective==
Learn how to maintain an OpenWetWare Notebook.
Today's experiment consists on creating Gold NPs filaments for further study. So we're doing the same protocol as on the previous Gold NPs synthesis.


==Description==
==Protocol==
# Add experimental record here. Include what, how, and why...
# 1ml of BSA (15.4µM)
# 1ml of HAuCl4 (4.3mM)
# 8ml of water
# Heat at 80°C, remove the solution from the oven every 30 minutes and leave it at room temperature for 10 minutes


==Data==
==Results==
* Add data and results here...


==Notes==
After having left the solution at room temperature during several days, we don't observe purple fibers but some grey fibers.
This area is for any observations or conclusions that you would like to note.
It seems that the Gold didn't react and mix with proteins to forms Gold NPs. The grey fibers should be formed by BSA proteins.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


[[Category:Course]]
[[Category:Course]]

Revision as of 12:44, 31 January 2012

File:BDLlogo notext 1r.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Today's experiment consists on creating Gold NPs filaments for further study. So we're doing the same protocol as on the previous Gold NPs synthesis.

Protocol

  1. 1ml of BSA (15.4µM)
  2. 1ml of HAuCl4 (4.3mM)
  3. 8ml of water
  4. Heat at 80°C, remove the solution from the oven every 30 minutes and leave it at room temperature for 10 minutes

Results

After having left the solution at room temperature during several days, we don't observe purple fibers but some grey fibers. It seems that the Gold didn't react and mix with proteins to forms Gold NPs. The grey fibers should be formed by BSA proteins.