User:Floriane Briere/Notebook/CHEM-496/2011/10/25: Difference between revisions
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==Objective== | ==Objective== | ||
Today's experiment consists on performing a gel electrophoresis on MPB-intein fusion which have been expressed and purified by groups 2 and 3. This experiment will help us to determine whether or not the expressions and purifications worked well. | |||
To do so, we are going to run a SDS-PAGE using a 12% discontinuous polyacrylamide gel. | |||
# The SDS-PAGE technique permits to separate proteins according to their mass. | |||
==Description== | ==Description== |
Revision as of 09:41, 25 October 2011
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ObjectiveToday's experiment consists on performing a gel electrophoresis on MPB-intein fusion which have been expressed and purified by groups 2 and 3. This experiment will help us to determine whether or not the expressions and purifications worked well. To do so, we are going to run a SDS-PAGE using a 12% discontinuous polyacrylamide gel.
Description
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