User:Floriane Briere/Notebook/CHEM-496/2011/10/25: Difference between revisions

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==Objective==
==Objective==
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Today's experiment consists on performing a gel electrophoresis on MPB-intein fusion which have been expressed and purified by groups 2 and 3. This experiment will help us to determine whether or not the expressions and purifications worked well.
 
To do so, we are going to run a SDS-PAGE using a 12% discontinuous polyacrylamide gel.
 
# The SDS-PAGE technique permits to separate proteins according to their mass.


==Description==
==Description==

Revision as of 09:41, 25 October 2011

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Objective

Today's experiment consists on performing a gel electrophoresis on MPB-intein fusion which have been expressed and purified by groups 2 and 3. This experiment will help us to determine whether or not the expressions and purifications worked well.

To do so, we are going to run a SDS-PAGE using a 12% discontinuous polyacrylamide gel.

  1. The SDS-PAGE technique permits to separate proteins according to their mass.

Description

  1. Add experimental record here. Include what, how, and why...

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.