User:Floriane Briere/Notebook/CHEM-496/2011/11/01: Difference between revisions

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* PCR: we are using the same protocol as on the 20th of September
* PCR: we are using the same protocol as on the 20th of September


==Data==
==Results==
* Add data and results here...
 
In tube 1, we obtained an incolore solution with a black/dark purple aggregate.
In tube 2, nothing happened, the solution remained incolore and no fibers or filaments was formed.


==Notes==
==Notes==

Revision as of 09:49, 2 November 2011

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Objective

Today we are going to conduct two different experiments:

  1. We are going to synthesize some Gold NPs again (tube 1) and we are going to investigate how the protein aggregate forms (tube 2; we are using an acidic solution to test if this property is responsible for the formation of the aggregate; the HAuCl4 is also an acidic solution).
  2. We are going to conduct a PCR again to produce some mutate proteins.

Protocol

  • Gold NPs synthesis.
  1. Tube 1 (in this specific order): add 1ml of BSA stock solution (15.5µM) + 1ml of HAuCl4 stock solution (2.9mM) + 8ml of water
  2. Tube 2 (in this specific order): add 1ml of HCl stock solution (3m) + 1ml of BSA stock solution (15.5µM) + 8ml of water
  • PCR: we are using the same protocol as on the 20th of September

Results

In tube 1, we obtained an incolore solution with a black/dark purple aggregate. In tube 2, nothing happened, the solution remained incolore and no fibers or filaments was formed.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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