User:Floriane Briere/Notebook/CHEM-496/2012/02/01

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Objective

Today's objective is to synthesize Gold NPs; we want to obtain a purple solution and fibers using Gold/BSA ratio we determined last semester. These solutions will then be used next week to test the binding of the dye.

Protocol

  • Gold/BSA ratio = 70
  1. Solution A: 0.042mL of HAuCl4 (2.9mM) + 0.1mL of BSA (17.7µM) + 0.85mL of water (2hours in the heatblock at 80°C)
  2. Solution C: 0.42mL of HAuCl4 (2.9mM) + 1mL of BSA (17.7µM) + 8.5mL of water (2hours in the oven at 80°C)
  • Gold/BSA ratio = 166
  1. Solution B: 0.1mL of HAuCl4 (2.9mM) + 0.1mL of BSA (17.7µM) + 0.8mL of water (2hours in the heatblock at 80°C)
  2. Solution D: 1mL of HAuCl4 (2.9mM) + 1mL of BSA (17.7µM) + 8mL of water (2hours in the oven at 80°C)

Data

  • For the heatblock experiments, we obtained:
  1. Some purple fibers into a clear solution for the 166 ratio(B)
  2. A purple solution for the 70 ratio (A)

  • For the oven experiments, we obtained:
  1. A purple solution for the 70 ratio (C)
  2. Some dark purple/black fibers into a clear solution for the 166 ratio (D)

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

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