User:Floriane Briere/Notebook/CHEM-496/2012/02/01

From OpenWetWare

< User:Floriane Briere | Notebook | CHEM-496 | 2012 | 02
Revision as of 16:29, 1 February 2012 by Floriane Briere (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Chem-496 Main project page
Previous entry      Next entry


Objective

Today's objective is to synthesize Gold NPs; we want to obtain a purple solution and fibers using Gold/BSA ratio we determined last semester. These solutions will then be used next week to test the binding of the dye.

Protocol

  • Gold/BSA ratio = 70
  1. Solution A: 0.042mL of HAuCl4 (2.9mM) + 0.1mL of BSA (17.7µM) + 0.85mL of water (2hours in the heatblock at 80°C)
  2. Solution C: 0.42mL of HAuCl4 (2.9mM) + 1mL of BSA (17.7µM) + 8.5mL of water (2hours in the oven at 80°C)
  • Gold/BSA ratio = 166
  1. Solution B: 0.1mL of HAuCl4 (2.9mM) + 0.1mL of BSA (17.7µM) + 0.8mL of water (2hours in the heatblock at 80°C)
  2. Solution D: 1mL of HAuCl4 (2.9mM) + 1mL of BSA (17.7µM) + 8mL of water (2hours in the oven at 80°C)

Data

  • For the heatblock experiments, we obtained:
  1. Some purple fibers into a clear solution for the 166 ratio(B)
  2. A purple solution for the 70 ratio (A)

Image:1feb - Gold NPs results.jpg

  • For the oven experiments, we obtained:
  1. A purple solution for the 70 ratio (C)
  2. Some dark purple/black fibers into a clear solution for the 166 ratio (D)

Image:1feb - Gold NPs results bis.jpg

Personal tools