User:Floriane Briere/Notebook/CHEM-496/2012/02/15

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Objective

Today's objective is to perform a dialysis using the solution we made yesterday. The reaction has been running for 24hours in the shaker, allowing the dye to tag our BSA peptides by binding unprotonated lysine. The dialysis is going to allow us to remove the excess of dye which hasn't been able to react with our Gold NPs. This step permits us to purify our solution so that we'll then be able to quantify labelled NPs using fluorescence spectroscopy. The first of our experiment is to lower the pH of our solution so that the reaction stops; to do so, we have to neutralize Tris molecules by using HCl (we have to add an amount of HCl which is equal to the amount of Tris we added yesterday).

Description

Data

Calculations for the volume of HCl needed to neutralize Tris.

Concentration of Tris buffer = 50mM Concentration of HCl solution = 0.948M

  1. Calculations for the control solution (tube 1)

Volume of Tris buffer = 321µL => Number of mole of Tris = 321 x 10^-6 x 50 x 10^-3 = 16µM => Volume of HCl solution to add = (1.6 x 10^-5)/0.948 = 16.8µL

  1. Calculations for the 166 ratio solution (tube 2)

Volume of Tris buffer = 1.5mL => Number of mole of Tris = 1.5 x 10^-3 x 50 x 10^-3 = 75µM => Volume of HCl solution to add = (7.5 x 10^-5)/0.948 = 79µL

  1. Calculations for the 70 ratio solution (tube 3).

Volume of Tris buffer = 600µL => Number of mole of Tris = 600 x 10^-6 x 50 x 10^-3 = 32µM => Volume of HCl solution to add = (3.2 x 10^-5)/0.948 = 32µL

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

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