User:Floriane Briere/Notebook/CHEM-496/2012/03/06: Difference between revisions
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==Objective== | ==Objective== | ||
Today's objective is to perform the first part of the tagging reaction which will be | Today's objective is to perform the first part of the tagging reaction which will be finished tomorrow so that the dialysis will be performed during the spring break week. So, we are going to use the 3 same solutions (70 ratio, 166 ratio and BSA/HCl control) and labelled them with the same dye. Then, after the spring break will perform fluorescence spectra to check if our results can be reproduced or enhanced. | ||
==Protocol== | ==Protocol== | ||
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# Add to each solution 160µL of the dye solution | # Add to each solution 160µL of the dye solution | ||
# Incubation at room temperature for 24 hours | # Incubation at room temperature for 24 hours | ||
[[Category:Course]] | [[Category:Course]] |
Revision as of 12:06, 30 April 2012
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ObjectiveToday's objective is to perform the first part of the tagging reaction which will be finished tomorrow so that the dialysis will be performed during the spring break week. So, we are going to use the 3 same solutions (70 ratio, 166 ratio and BSA/HCl control) and labelled them with the same dye. Then, after the spring break will perform fluorescence spectra to check if our results can be reproduced or enhanced. ProtocolWe are going to use solutions prepared on the 8th of february.
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