User:Floriane Briere/Notebook/CHEM-496/2012/03/07: Difference between revisions
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* Run the dialysis into water, water is going tobe changed several from 3/8 to the 3/20. | * Run the dialysis into water, water is going tobe changed several from 3/8 to the 3/20. | ||
The dialysis was performed using dialysis tubing in regenerated cellulose (Fisherbrand). | The dialysis was performed using dialysis tubing in regenerated cellulose (Fisherbrand) and in the dark (covered by foil). | ||
==Data== | ==Data== |
Revision as of 11:59, 7 March 2012
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ObjectiveToday's objective is to prepare the dialysis which will be running during the spring break (until 3/20/12). We're going to change the protocol compare to what we made last time. We're going to add HCl (1M) until the solution turns pink/purple to stop the tagging reaction, and we're not goint to add Tris buffer to higher the pH. We'll add some Tris buffer after the dialysis, just before performing the fluorescence spectrum, and until the solution turns blue again. We should have to add less Tris buffer than last time because the pH has not been lower as much as last time (and the solutions' color is due to the pH of the solution). Protocol
The dialysis was performed using dialysis tubing in regenerated cellulose (Fisherbrand) and in the dark (covered by foil). Data
NotesThis area is for any observations or conclusions that you would like to note.
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