User:Floriane Briere/Notebook/CHEM-496/2012/03/20: Difference between revisions
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# 166 ratio with dye (prepared last 2 weeks) | # 166 ratio with dye (prepared last 2 weeks) | ||
# BSA/HCl with dye (prepared last 2 weeks) | # BSA/HCl with dye (prepared last 2 weeks) | ||
We are going to use the protocol we established on the 2/22 to 'activate' the dye; so we are going to add Tris buffer until every solution turns blue. | |||
==Protocol== | ==Protocol== | ||
* Preparation of the BSA/HCl control. | * Preparation of the BSA/HCl control. | ||
# Add 1mL of HCl (2.84mM) + 1mL BSA + 8mL water | # Add 1mL of HCl (2.84mM) + 1mL BSA(17.7µM) + 8mL water | ||
# Put into the oven for 2 hours | # Put into the oven for 2 hours | ||
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# Centrifugation (5 minutes at 13200rpm) of the 166 ratio with dye solution | # Centrifugation (5 minutes at 13200rpm) of the 166 ratio with dye solution | ||
== | ==Results== | ||
* UV spectroscopy results. | |||
[[Image:20mar - UV curve.jpg]] | |||
[[Image:20mar - UV curve zoom.jpg]] | |||
[[ | |||
* Fluorescence spectroscopy results. | |||
[[Image:20mar - fluorescence curve.jpg]] | |||
[[Category:Course]] | [[Category:Course]] |
Revision as of 16:45, 25 March 2012
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ObjectiveToday's objective is to perform the fluorescence and UV spectroscopy on our samples. We are going to perform our analysis on 6 different samples:
We are going to use the protocol we established on the 2/22 to 'activate' the dye; so we are going to add Tris buffer until every solution turns blue. Protocol
Results
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