User:Floriane Briere/Notebook/CHEM-496/2012/03/20: Difference between revisions

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# 166 ratio with dye (prepared last 2 weeks)
# 166 ratio with dye (prepared last 2 weeks)
# BSA/HCl with dye (prepared last 2 weeks)
# BSA/HCl with dye (prepared last 2 weeks)
# BSA/HCl control (prepared today)
We are going to use the protocol we established on the 2/22 to 'activate' the dye; so we are going to add Tris buffer until every solution turns blue.
# 166 ratio control (prepared 2/28)
# 70 ratio control (prepared 2/28)
The dye in solution control isn't going to be redo today; we're going to use data from 22/2.


==Protocol==
==Protocol==
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[[Image:20mar - fluorescence curve.jpg]]
[[Image:20mar - fluorescence curve.jpg]]
==Notes==
This area is for any observations or conclusions that you would like to note.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
[[Category:Course]]
[[Category:Miscellaneous]]


[[Category:Course]]
[[Category:Course]]

Revision as of 16:45, 25 March 2012

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Objective

Today's objective is to perform the fluorescence and UV spectroscopy on our samples. We are going to perform our analysis on 6 different samples:

  1. 70 ratio with dye (prepared last 2 weeks)
  2. 166 ratio with dye (prepared last 2 weeks)
  3. BSA/HCl with dye (prepared last 2 weeks)

We are going to use the protocol we established on the 2/22 to 'activate' the dye; so we are going to add Tris buffer until every solution turns blue.

Protocol

  • Preparation of the BSA/HCl control.
  1. Add 1mL of HCl (2.84mM) + 1mL BSA(17.7µM) + 8mL water
  2. Put into the oven for 2 hours
  • Preparation of samples with dye
  1. After dialysis, we obtained:
    1. The 70 ratio with dye was a clear solution with some purple aggregats/beads
    2. The 166 ratio with dye was a clear solution with some purple aggregats/fibers
    3. The BSA/HCl with dye was a pink solution with some pink beads
  2. Add Tris buffer until the solution turns blue
  3. Vortex the samples: the 70 ratio and BSA/HCl solutions dissolved whereas 166 ratio didn't
  4. Centrifugation (5 minutes at 13200rpm) of the 166 ratio with dye solution

Results

  • UV spectroscopy results.

  • Fluorescence spectroscopy results.

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