User:Floriane Briere/Notebook/CHEM-496/2012/03/27: Difference between revisions
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==Notes== | ==Notes== | ||
* We noticed initial solutions had similar pH except for the 70 ratio with. So, we decided to increase the pH of this solution to level pH value between the 3 different solutions. | |||
* By comparing initial and second solutions pH value, we noticed that the pH of initial solutions was higher (around 8.35) than pH of second solutions (around 8.1). This difference could explain the fact that we haven't been able to reproduce our data. So, we increased pH of second solutions to match initial solutions pH. Then, we performed fluorescence spectrum and noticed that fluorescence curves are related to pH. | |||
* We also noticed that pH of second solutions evolved during the week; pH value were different from one we measured last week. This difference can be explained by contaminants (water used to clean tubes, pH meter), or by the fact that a reaction is occuring into our solutions and affect pH value. The pHmeter has been calibrated before each set of measure, so the difference shouldn't come from the instrument. | |||
Revision as of 11:24, 27 March 2012
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ObjectiveLast week, we haven't been able to reproduce data we collected in fabruary for fluorescence spectrum; especially, the 166 ratio with dye solution gives very different results (it was the lowest curve in february but the highest last week). To try to explain this difference, we are going to measure pH of initial solutions (made in february) and compare it with the pH of second solutions (made in march). By comparing curves and pH, we are going to see whether or not it can explain the non reproducibility of our results.
We still miss the following data:
Protocol
Results
Notes
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