User:Floriane Briere/Notebook/CHEM-496/2012/03/27: Difference between revisions
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# Increase the pH by adding Tris buffer: | # Increase the pH by adding Tris buffer: | ||
## 70 ratio with dye: add 6 drops, final pH = 8.29 | ## 70 ratio with dye: add 6 drops, final pH = 8.29 | ||
## | ## 166 ratio with dye: add 3 drops, final pH = 8.41 | ||
## | ## BSA/HCl ratio with dye: add 7 drops, final pH = 8.28 | ||
* Fluorescence spectrum of 3/21 solutions (2nd solutions). | * Fluorescence spectrum of 3/21 solutions (2nd solutions). |
Revision as of 10:04, 10 April 2012
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ObjectiveLast week, we haven't been able to reproduce data we collected in fabruary for fluorescence spectrum; especially, the 166 ratio with dye solution gives very different results (it was the lowest curve in february but the highest last week). To try to explain this difference, we are going to measure pH of initial solutions (made in february) and compare it with the pH of second solutions (made in march). By comparing curves and pH, we are going to see whether or not it can explain the non reproducibility of our results.
We still miss the following data:
Protocol
Results
Notes
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