User:Floriane Briere/Notebook/CHEM-496/2012/03/27

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Objective

Protocol

  • 2/21 solutions(initial solutions).
  1. Measure the initial pH:
    1. 70 ratio with dye: initial pH = 8.12
    2. 166 ratio with dye: initial pH = 8.43
    3. BSA/HCl with dye: initial pH = 8.3
  2. Increase the pH by adding Tris buffer:
    1. 70 ratio with dye: add 10 drops, final pH = 8.30
  • 3/21 solutions (2nd solutions).
  1. Measure the initial pH:
    1. 70 ratio with dye: initial pH = 8.00 (last week pH = 8.07)
    2. 166 ratio with dye: initial pH = 8.35 (last week pH = 8.13)
    3. BSA/HCl with dye: initial pH = 7.98 (last week pH = 8.09)
  2. Increase the pH by adding Tris buffer:
    1. 70 ratio with dye: add 6 drops, final pH = 8.29
    2. 70 ratio with dye: add 3 drops, final pH = 8.41
    3. 70 ratio with dye: add 7 drops, final pH = 8.28
  • Fluorescence spectrum of 3/21 solutions (2nd solutions).
  1. Centrifugation (5 minutes, 13200rpm)
  2. Take spectrum: excitation = 600nm; emission = 620 to 800nm
  • Fluorescence spectrum of 2/21 solutions (initial solutions).
  1. Take spectrum: excitation = 600nm; emission = 620 to 800nm
  • Fluorescence spectrum of control solutions.
  1. 166 ratio alone: prepared on the 2/28
  2. 70 ratio alone: prepared on the 2/28
  3. BSA/HCl alone: prepared on the 3/20

Results

  • Fluorescence spectrum: second solutions.

Notes

This area is for any observations or conclusions that you would like to note.


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