User:Floriane Briere/Notebook/CHEM-496/2012/04/11: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 13: Line 13:
Today's objective is to analyze precipitates that formed after performing the first and second dye reactions using dry fluorescence spectroscopy. These analyses will also us to test the presence/absence of dye in the pellet; the presence of dye in the pellet could lead to falsify the dye/BSA ratio calculations.
Today's objective is to analyze precipitates that formed after performing the first and second dye reactions using dry fluorescence spectroscopy. These analyses will also us to test the presence/absence of dye in the pellet; the presence of dye in the pellet could lead to falsify the dye/BSA ratio calculations.


==Description==
==Protocol==
 
# Filled plastic centrifuge tubes with the 166 and 70 Au/BSA ratio dye solutions, and the BSA/HCl dye solutions from both the first and second dye reactions.
# Centrifuged all solutions ( 13500 rpm, 5 min).
# Poured supernatant from solutions into their respective tubes leaving only the pellets in the plastic centrifuge tubes.
# Scraped pellet into separate test tubes.
# Pellet was placed onto silica plate, which was prepared for dry fluorescence.
# Take fluorescence spectra of sample.


==Data==
==Data==

Revision as of 13:13, 30 April 2012

Chem-496 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Objective

Today's objective is to analyze precipitates that formed after performing the first and second dye reactions using dry fluorescence spectroscopy. These analyses will also us to test the presence/absence of dye in the pellet; the presence of dye in the pellet could lead to falsify the dye/BSA ratio calculations.

Protocol

  1. Filled plastic centrifuge tubes with the 166 and 70 Au/BSA ratio dye solutions, and the BSA/HCl dye solutions from both the first and second dye reactions.
  2. Centrifuged all solutions ( 13500 rpm, 5 min).
  3. Poured supernatant from solutions into their respective tubes leaving only the pellets in the plastic centrifuge tubes.
  4. Scraped pellet into separate test tubes.
  5. Pellet was placed onto silica plate, which was prepared for dry fluorescence.
  6. Take fluorescence spectra of sample.

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Floriane_Briere/Notebook/CHEM-496/2012/04/11&oldid=599962"