User:Guido/Notes/July 07: Difference between revisions
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(New page: == July 3, 2007 == * Do a culture pcr from last recombination of specR to replace MutS ** 3 μl culture ** 5 μl taq buffer ** 1 μl f-primer ** 1 μl r-primer ** .5 μl taq ...) |
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== July 3, 2007 == | == July 3, 2007 == | ||
* Do a culture pcr from last recombination of specR to replace MutS | * Do a culture pcr from last recombination of specR to replace MutS | ||
** 3 μl culture | ** 3 μl culture | ||
| Line 13: | Line 13: | ||
** 50 μl reaction | ** 50 μl reaction | ||
* 72 5 min | * 94 4 min | ||
* 4 forever | |||
* 94 30 sec | |||
* 53 30 sec | |||
* 72 2 min | |||
* 94 30 sec | |||
* 53 30 sec | |||
* 72 2 min | |||
* 72 5 min | |||
* 4 forever | |||
Revision as of 07:37, 3 July 2007
July 3, 2007
- Do a culture pcr from last recombination of specR to replace MutS
- 3 μl culture
- 5 μl taq buffer
- 1 μl f-primer
- 1 μl r-primer
- .5 μl taq
- 39.5 dH20
- 50 μl reaction
* 94 4 min * 94 30 sec * 53 30 sec * 72 2 min * 94 30 sec * 53 30 sec * 72 2 min * 72 5 min * 4 forever
