User:Guido/Notes/July 07

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(New page: == July 3, 2007 == * Do a culture pcr from last recombination of specR to replace MutS ** 3 μl culture ** 5 μl taq buffer ** 1 μl f-primer ** 1 μl r-primer ** .5 μl taq ...)
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Revision as of 10:36, 3 July 2007

July 3, 2007

  • Do a culture pcr from last recombination of specR to replace MutS
    • 3 μl culture
    • 5 μl taq buffer
    • 1 μl f-primer
    • 1 μl r-primer
    • .5 μl taq
    • 39.5 dH20
    • 50 μl reaction
  • 94 4 min
  • 94 30 sec
  • 53 30 sec
  • 72 2 min
  • 94 30 sec
  • 53 30 sec
  • 72 2 min
  • 72 5 min
  • 4 forever
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