User:Guido/Notes/July 07
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(New page: == July 3, 2007 == * Do a culture pcr from last recombination of specR to replace MutS ** 3 μl culture ** 5 μl taq buffer ** 1 μl f-primer ** 1 μl r-primer ** .5 μl taq ...)
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Revision as of 10:36, 3 July 2007
July 3, 2007
- Do a culture pcr from last recombination of specR to replace MutS
- 3 μl culture
- 5 μl taq buffer
- 1 μl f-primer
- 1 μl r-primer
- .5 μl taq
- 39.5 dH20
- 50 μl reaction
- 94 4 min
- 94 30 sec
- 53 30 sec
- 72 2 min
- 94 30 sec
- 53 30 sec
- 72 2 min
- 72 5 min
- 4 forever
