User:Guido/Notes/July 07

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(New page: == July 3, 2007 == * Do a culture pcr from last recombination of specR to replace MutS ** 3 μl culture ** 5 μl taq buffer ** 1 μl f-primer ** 1 μl r-primer ** .5 μl taq ...)
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== July 3, 2007 ==
== July 3, 2007 ==
* Do a culture pcr from last recombination of specR to replace MutS
* Do a culture pcr from last recombination of specR to replace MutS
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** 3 μl culture
** 3 μl culture
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** 50  μl reaction
** 50  μl reaction
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* 94  4 min
 
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* 94  30 sec 
 
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* 53  30 sec
 
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* 72  2 min
 
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* 94  30 sec
 
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* 53  30 sec
 
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* 72  2 min
 
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* 72  5 min
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* 94  4 min
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* 4 forever
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 +
* 94  30 sec 
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* 53  30 sec
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* 72  2 min
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* 94  30 sec
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* 53  30 sec
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* 72  2 min
 +
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* 72  5 min
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* 4 forever

Revision as of 10:37, 3 July 2007

July 3, 2007

  • Do a culture pcr from last recombination of specR to replace MutS


    • 3 μl culture
    • 5 μl taq buffer
    • 1 μl f-primer
    • 1 μl r-primer
    • .5 μl taq
    • 39.5 dH20
    • 50 μl reaction


* 94  4 min

* 94  30 sec  
* 53  30 sec
* 72  2 min

* 94  30 sec
* 53  30 sec
* 72  2 min

* 72   5 min
* 4 forever
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