User:Guido/Notes/July 07: Difference between revisions
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Revision as of 07:45, 3 July 2007
July 3, 2007
- Do a culture pcr from last recombination of specR to replace MutS
- 3 μl culture
- 5 μl taq buffer
- 1 μl f-primer
- 1 μl r-primer
- .5 μl taq
- 39.5 dH20
- 50 μl reaction
* 94 4 min * 94 30 sec * 53 30 sec * 72 2 min * 94 30 sec * 53 30 sec * 72 2 min * 72 5 min * 4 forever
