User:Guido/Notes/July 07

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Current revision (11:35, 3 July 2007) (view source)
 
Line 6: Line 6:
** 3 &mu;l culture
** 3 &mu;l culture
-
** 5 &mu;l taq buffer
 
** 1 &mu;l f-primer
** 1 &mu;l f-primer
** 1 &mu;l r-primer
** 1 &mu;l r-primer
-
** .5 &mu;l taq
+
** 45 &mu;l supermix
-
** 39.5 dH20
+
** 50  &mu;l reaction
** 50  &mu;l reaction
-
  * 94 4 min
+
  * 95 10 min
   
   
-
  * 94 30 sec   
+
  * 95 30 sec   
-
  * 53 30 sec
+
  * 51 30 sec
  * 72  2 min
  * 72  2 min
 +
* X30
   
   
-
* 94  30 sec
+
  * 72  7 min
-
* 53  30 sec
+
-
* 72  2 min
+
-
+
-
  * 72  5 min
+
  * 4 forever
  * 4 forever

Current revision

July 3, 2007

  • Do a culture pcr from last recombination of specR to replace MutS


    • 3 μl culture
    • 1 μl f-primer
    • 1 μl r-primer
    • 45 μl supermix
    • 50 μl reaction 


* 95  10 min

* 95  30 sec  
* 51  30 sec
* 72  2 min
* X30

* 72   7 min
* 4 forever
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