User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/08/31: Difference between revisions

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Revision as of 11:15, 1 September 2016

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objectives

  1. Run fluorescence data for lysozyme at pH=4
  2. Create stock solutions for use on 09062016


Description

Fluorescence

  1. Solutions made using measurements below
  2. Fluorescence data run every 5 minutes for 3 hours

Stock Solutions for 09/06/2016 Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.


Data

Fluorescence

  1. Solution Measurements
    1. Lysosome Stock Solution
      1. 8.88 mg of lysozyme with water in 10.0 mL volumetric flask
      2. MW = 14307 g/mol
      3. concentration = 62.1 μM
    2. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total; pH 4
      1. 225 μL AuCl3
      2. 300 μL 1M HCl
      3. 604 μL Lysozyme
      4. 1871 μL Water

Solution Prep

Lysozyme UVVis solution measurement 0 mM fructose

pH HCl (μL) NaOHc (μL) NaOHd (μL) AuCl3 (μL) Fructose (μL) Lysozyme (μL) Water (μL)
4 500 0 0 300 0 252 3949
5 50 0 0 300 0 252 4399
6 5 0 0 300 0 252 4444
7 0 0 0 300 0 252 4449
8 0 0 5 300 0 252 4444
9 0 0 50 300 0 252 4399
10 0 0 500 300 0 252 3949
11 0 5 0 300 0 252 4444
12 0 50 0 300 0 252 4399

Lysozyme UVVis solution measurement .0625 mM fructose

pH HCl (μL) NaOHc (μL) NaOHd (μL) AuCl3 (μL) Fructose (μL) Lysozyme (μL) Water (μL)
4 500 0 0 300 24 252 3925
5 50 0 0 300 24 252 4375
6 5 0 0 300 24 252 4420
7 0 0 0 300 24 252 4425
8 0 0 5 300 24 252 4420
9 0 0 50 300 24 252 4375
10 0 0 500 300 24 252 3925
11 0 5 0 300 24 252 4420
12 0 50 0 300 24 252 4375
  • Matt Hartings 14:04, 1 September 2016 (EDT): Your sample prep didn't work. See my lab notebook page here for a description of why and how I remade your samples.
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