User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/18: Difference between revisions

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==Description==
==Description==
#Test solutions made according to below concentrations under Data.  
#Test solutions made according to below concentrations under Data.  
#On 9/21, solutions were placed into the oven at 80°C for 4 hours.  
#On 10-12-16, solutions were placed into the oven at 80°C for 4 hours.  
#UV-vis data was run on each of the 18 samples of different pHs and fructose concentrations.  
#UV-vis data was run on each of the 18 samples of different pHs and fructose concentrations.  
#After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H<sub>2</sub>O, twice with HCl, and twice more with H<sub>2</sub>O. The cuvette was then dried thoroughly.  
#After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H<sub>2</sub>O, twice with HCl, and twice more with H<sub>2</sub>O. The cuvette was then dried thoroughly.  
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[[Image:09.27.16 abs v wave lys fruc.75.png|thumb|upright=4.5]] <br>
[[Image:101816 Abs v Wave Fruc .125mM.pngthumb|upright=4.5]] <br>
'''Figure 1'''  Absorbance v wavelength of lysozyme in 0.75 mM concentration of fructose  
'''Figure 1'''  Absorbance v wavelength of lysozyme in 0.125 mM concentration of fructose  


[[Image:09.27.16 abs v pH lys fruc.75.png|thumb|upright=4.5]] <br>
[[Image:101816 Abs v pH Fruct .125mM.png|thumb|upright=4.5]] <br>
'''Figure 2''' Absorbance at 527 nm at various pH's in 0.75 mM concentration of fructose
'''Figure 2''' Absorbance at 279 nm at various pH's in 0.125 mM concentration of fructose


[[Image:091416 Abs v Wave Fruc25.png|thumb|upright=4.5]] <br>
[[Image:101816 Abs v Wave Fruc .25mM.png|thumb|upright=4.5]] <br>
'''Figure 3''' Absorbance v wavelength of lysozyme in 0.25 mM concentration of fructose  
'''Figure 3''' Absorbance v wavelength of lysozyme in 0.25 mM concentration of fructose  


[[Image:09.27.16 abs v wave lys fruc1.25.png|thumb|upright=4.5]] <br>
[[Image:101816 Abs v pH Fruc .25mM.png|thumb|upright=4.5]] <br>
'''Figure 4''' Absorbance at 527 nm at various pH's in 1.25 mM concentration of fructose <br>
'''Figure 4''' Absorbance at 279 nm at various pH's in .25 mM concentration of fructose <br>


==Notes==  
==Notes==  

Revision as of 16:00, 25 October 2016

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Objectives

  1. Run UV-vis for 18 samples with lysozyme as the protein. Full concentration details can be found below under Data.

Description

  1. Test solutions made according to below concentrations under Data.
  2. On 10-12-16, solutions were placed into the oven at 80°C for 4 hours.
  3. UV-vis data was run on each of the 18 samples of different pHs and fructose concentrations.
  4. After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H2O, twice with HCl, and twice more with H2O. The cuvette was then dried thoroughly.

Data

UV-vis solutions

Table 1 UV-vis solutions made for [Fructose]=.125mM with silver


Table 2 UV-vis solutions made for [Fructose]=.25mM with silver


File:101816 Abs v Wave Fruc .125mM.pngthumb
Figure 1 Absorbance v wavelength of lysozyme in 0.125 mM concentration of fructose


Figure 2 Absorbance at 279 nm at various pH's in 0.125 mM concentration of fructose


Figure 3 Absorbance v wavelength of lysozyme in 0.25 mM concentration of fructose


Figure 4 Absorbance at 279 nm at various pH's in .25 mM concentration of fructose

Notes

All Figures in the above data are baseline and water corrected.
After taking the UV-vis samples out of the 80°C oven, pH 11 for both Fructose concentrations showed a precipitate form. pH 12 did not show this precipitate.


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