User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/19: Difference between revisions

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==Entry title==
==Objective==
* Insert content here...
#Run Ocean Optics for Lysozyme protein with a pH of 6.  
 
==Description==
#Make solution for cuvette. Concentrations can be found in the '''Data''' section below.
#Run Ocean Optics data every two minutes for 3 hours.
 
==Data==
<u> Stock solutions </u>
# Lysozyme
## concentration = 210 μM
# AuCl<sub>3</sub>
## concentration = 4.27 mM
 
<u>Instructions for making sample for ocean optics</u>
# pH 6
## Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
### 176 μL AuCl<sub>3</sub>
### 3 μL 1M HCl
### 179 μL Lysozyme
### 2642 μL Water
 
[[Image:092816 Wave pH 6.png|thumb|upright=4.0]]<br>
'''Figure 1''' Values taken with lysozyme at a pH of 6
 
[[Image:092816 Abs pH 6.png|thumb|upright=4.0]]<br>
'''Figure 2''' Values taken with lysozyme at a pH of 6
 
[[Image:092816 Abs Wave pH 6.png|thumb|upright=4.0]]<br>
'''Figure 3''' Absorbance v wavelength Ocean Optics data for lysozyme at a pH of 6. Values shown every 30 minutes. In addition, values shown at t=0-4 min t=32-34 min due to the large jump observed in '''Figure 1'''
 




Revision as of 10:19, 25 October 2016

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Objective

  1. Run Ocean Optics for Lysozyme protein with a pH of 6.

Description

  1. Make solution for cuvette. Concentrations can be found in the Data section below.
  2. Run Ocean Optics data every two minutes for 3 hours.

Data

Stock solutions

  1. Lysozyme
    1. concentration = 210 μM
  2. AuCl3
    1. concentration = 4.27 mM

Instructions for making sample for ocean optics

  1. pH 6
    1. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
      1. 176 μL AuCl3
      2. 3 μL 1M HCl
      3. 179 μL Lysozyme
      4. 2642 μL Water

Figure 1 Values taken with lysozyme at a pH of 6


Figure 2 Values taken with lysozyme at a pH of 6


Figure 3 Absorbance v wavelength Ocean Optics data for lysozyme at a pH of 6. Values shown every 30 minutes. In addition, values shown at t=0-4 min t=32-34 min due to the large jump observed in Figure 1



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