User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/26: Difference between revisions
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== | ==Objectives== | ||
#Run UV-vis for 18 samples with lysozyme as the protein. Full concentration details can be found below under Data. | |||
==Description== | |||
#Test solutions made according to below concentrations under Data. | |||
#On 9/21, solutions were placed into the oven at 80°C for 4 hours. | |||
#UV-vis data was run on each of the 18 samples of different pHs and fructose concentrations. | |||
#After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H<sub>2</sub>O, twice with HCl, and twice more with H<sub>2</sub>O. The cuvette was then dried thoroughly. | |||
==Data== | |||
'''Solution concentrations'''<br> | |||
[[Image:092716 UVvis sol Lys fruc75.png]]<br> | |||
'''Table 1''' UV-vis solutions made for [Fructose]=.75mM | |||
[[Image:092716 UVvis Lys fruc125.png]]<br> | |||
'''Table 2''' UV-vis solutions made for [Fructose]=1.25mM | |||
[[Image:09.27.16 abs v wave lys fruc.75.png|thumb|upright=4.5]] <br> | |||
'''Figure 1''' Absorbance v wavelength of lysozyme in 0.75 mM concentration of fructose | |||
[[Image:09.27.16 abs v pH lys fruc.75.png|thumb|upright=4.5]] <br> | |||
'''Figure 2''' Absorbance at 527 nm at various pH's in 0.75 mM concentration of fructose | |||
[[Image:091416 Abs v Wave Fruc25.png|thumb|upright=4.5]] <br> | |||
'''Figure 3''' Absorbance v wavelength of lysozyme in 0.25 mM concentration of fructose | |||
[[Image:09.27.16 abs v wave lys fruc1.25.png|thumb|upright=4.5]] <br> | |||
'''Figure 4''' Absorbance at 527 nm at various pH's in 1.25 mM concentration of fructose <br> | |||
==Notes== | |||
All Figures in the above data are baseline and water corrected. <br> | |||
After taking the UV-vis samples out of the 80°C oven, pH 11 for both Fructose concentrations showed a precipitate form. pH 12 did not show this precipitate. | |||
Revision as of 10:17, 25 October 2016
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Objectives
Description
DataSolution concentrations
Figure 1 Absorbance v wavelength of lysozyme in 0.75 mM concentration of fructose Figure 2 Absorbance at 527 nm at various pH's in 0.75 mM concentration of fructose Figure 3 Absorbance v wavelength of lysozyme in 0.25 mM concentration of fructose Figure 4 Absorbance at 527 nm at various pH's in 1.25 mM concentration of fructose NotesAll Figures in the above data are baseline and water corrected.
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