User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/11/16

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Objectives

  1. Run UV-vis for 20 samples with lysozyme as the protein. Full concentration details can be found below under Data.

Description

  1. Test solutions made according to below concentrations under Data.
  2. On 11/15, solutions were placed into the oven at 80°C for 4 hours.
  3. UV-vis data was run on each of the 20 samples of different pHs and fructose concentrations.
  4. After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H2O, twice with HCl, and twice more with H2O. The cuvette was then dried thoroughly.

Data

Solution concentrations
UV-vis solutions for 11.16.2016


Figure 1 Absorbance v wavelength of lysozyme in 0.75 mM concentration of fructose; Ag


Figure 2 Absorbance at 272 nm at various pH's in 0.75 mM concentration of fructose; Ag


Figure 3 Absorbance v wavelength of lysozyme in 1.25 mM concentration of fructose; Ag


Figure 4 Absorbance at 280 nm at various pH's in 1.25 mM concentration of fructose; Ag


Figure 5 Absorbance v wavelength of lysozyme in 0.0 mM concentration of fructose; Au


Figure 6 Absorbance at 527 nm at various pH's in 0.0 mM concentration of fructose; Au


Figure 7 Absorbance v wavelength of lysozyme in 0.0625 mM concentration of fructose; Au


Figure 8 Absorbance at 527 nm at various pH's in 0.0625 mM concentration of fructose; Au

Notes

All Figures in the above data are baseline and water corrected.



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