User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/11/30: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2016/11/30 Entry for User:Hannah_Harvey/Notebook/CHEM-471_Fall_2016)
 
No edit summary
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Objectives==
* Insert content here...
#Run fluorescence data for lysozyme at pH=12
#Create stock solutions for use on 12.06.2016
 
==Description==
<u>Fluorescence</u>
#Solutions made using measurements below
#Fluorescence data run every 3 minutes for 3 hours
 
<u>Solutions for 11/16/2016</u><br>
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.
 
==Data==
 
[[Image:113016 Emis Time Fluor Lys pH12.png|thumb|upright=5.0]]<br>
'''Figure 1''' Maximum emission data in a solution of pH 12.<br>
 
[[Image:113016 Int Time Fluor Lys pH12.png|thumb|upright=5.0]]<br>
'''Figure 2''' Integrated fluorescence data in a solution of pH 12. <br>
 
[[Image:113016 Abs Wave Fluor Lys pH12.png|thumb|upright=5.0]]<br>
'''Figure 3''' Full emission spectra for important time points: t=0, 69, and every 30 min from 90-180 min. <br>
 
<u>'''Figure 3''' Explanation</u><br>
This data looks different  from the other runs up to this point.
 
<u>UV-vis solutions for 12.06.2016</u><br>
[[Image:UVvis Solutions 120616.png]]<br>
 
==Notes==
This pH should be run again with a lower concentration of the lysozyme because of the flattening out at Absorbance=1000. The "flattening" is likely due to the elimination of the interaction of gold and the tryptophan in the lysozyme protein. The lower concentration of lysozyme will allow us to visualize the true maximum absorbance level. This will also change the results shown in '''Figure 1'''. Currently, the graph is showing most of the maximum emission to be around 1000 due to this skewed data.  




Revision as of 13:30, 7 December 2016

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  1. Run fluorescence data for lysozyme at pH=12
  2. Create stock solutions for use on 12.06.2016

Description

Fluorescence

  1. Solutions made using measurements below
  2. Fluorescence data run every 3 minutes for 3 hours

Solutions for 11/16/2016
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.

Data


Figure 1 Maximum emission data in a solution of pH 12.


Figure 2 Integrated fluorescence data in a solution of pH 12.


Figure 3 Full emission spectra for important time points: t=0, 69, and every 30 min from 90-180 min.

Figure 3 Explanation
This data looks different from the other runs up to this point.

UV-vis solutions for 12.06.2016

Notes

This pH should be run again with a lower concentration of the lysozyme because of the flattening out at Absorbance=1000. The "flattening" is likely due to the elimination of the interaction of gold and the tryptophan in the lysozyme protein. The lower concentration of lysozyme will allow us to visualize the true maximum absorbance level. This will also change the results shown in Figure 1. Currently, the graph is showing most of the maximum emission to be around 1000 due to this skewed data.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Hannah_Harvey/Notebook/CHEM-471_Fall_2016/2016/11/30&oldid=967446"