User:Hannah T Sherman/Notebook/Biology 210 at AU

Image 1 lab 3.jpg"2/16/14" [[Image:Image 1 lab 3 File:Image 7 lab 3.jpeg Lab 3 Microbiology and Identifying Bacteria with DNA State the question/ problem /Objective: There are three objectives in the “Microbiology and Identifying Bacterial with DNA “ lab. The first objective of this lab is to allow use to better understand the characteristics of bacteria. The second that was given was observing antibiotic resistance. The third one addresses how species are identified by sequencing DNA. We fulfilled these three objectives in this lab by observing our ager plates from last weeks experiment and seeing how much growth it had and seeing the different between plates with resistance and without . Then taking a sample form a specific one and making a gram sample. Then by preparing DNA for PCR. Over the last two weeks the hey infusion has change how it smells and looks. This week when we observed how the hey infusion smelled it smell a lot less bitter and a lot more like a camp lake. The appearance. The hey infusion is a lot less cloudy and more clear. I am not completely sure why is smells less potent but maybe it is because it has finally settled or microorganisms in the culture the reason why it is so much more clear is because the particles in the infusion have settled. The different that is seen between the plats that has been seen between the Nutrient plats and the one that has antibiotics on it is to begin with there are smaller colonies or no colonies on the anti biotic plates. There was only one antibiotic plat that had growth and the growth was very different from the rest of the plats. The growth was yellow and all in one spot all together. This could indicate one of two things first that the growth grow all together because that is the only way it can grow on that plat ore that it only grow certain things one the plat. Tetracycline which is what is put on the anti bacteria plates affected the colonies size by causing no growth or only one large growth. The only one that we found was fusiturm on the 10-2 plat. Tetracycline works by being an inhibiter through diminishing the ability to produce certain vital proteins. This inhibits cholera , rickettsial infections, trachoma. psittacosis , brucellosis, tularemic and other bacterias. Record the specific steps you performed:

Start the lab by checking your hay infusion for one last time note any changes in the hay infusion. After that look at the agar plates from the week before see if there was any growth note the differences in growth between the ones that had antibiotic and antibiotic resistance. The next part of the experiment is to practice observing tiny cells. Start by obtaining a slide that contains different types and shapes of bacteria. Be sure to observe the stained parts of the slide and observe at 40X objective. Then continue by using the 100X oil immersion objective be sure to only use a very little bit of oil . Then be sure to clean the oil objective. After this each group will observe four samples of microorganisms. From the colonies that are observed choose one of the isolated ones. The isolated organism will be used for PCR and species identification.Each of of our sample we will make a wet mount and a gram stain. To make a wet mount use a clean loop and then put a group of water on the slide and then put a cover slip over the slide. Then make another slide doing the exact same thing minus the cover slip and then put a red circle around the sample. Then take the first slide and observe it under the microscope. This should be looked at at 10 X and 40 X. Make observations through it may be hard to see. Next you make a gram stain start by labeling the slide that you made before with the red circle. Once it is labeled heat fix it by passing it through a flam 3 times. Then cover the slide with crystal violet for one minute then rinse off with distilled water. Next cover it with iodine molder for a minute and rinse that off. Flood the slide for 10 to 20 seconds with 95% alcohol it is complete when it comes off colorless. For 20-30 seconds cover the slide with safranin stain and rinse gently. Blot excess water carefully with dry paper towel. Once finished look at it under the microscope at 40X and at the oil objective. Finally we start our PCR which is done by getting a colony of bacteria from the plates and putting it into 100 ul of water in a sterile tube. The it should be incubated for 10 mins 100C then centrifuge. Using 5ul of the supernatant in the PCR reaction. Next week these PCRs will be ran in the gel. 

Include all of your raw data : Table 1

Table 2

State conclusions and future plans: In this lab our objectives where to understand the characteristics of bacteria, observing antibiotic resistance, how species are identified by sequencing DNA. We fulfilled these objectives first by procedure one through 3 by observing the Ager plates that we did and see what growth had happened and what the growth looked like. For the most part the majority of the growth was on the Nutrient plates which is was I thought would be the result. Then we characterized three after making a gram stain of this fulfilled the objective of characterizing bacteria and observing antibiotic resistance. Procedure four started to fulfill the last objective by starting to isolate the DNA from the hey infusions. This lab helped use to better understand bacteria . Citation: tetracycline. (2014). In Encyclopaedia Britannica. Retrieved from http://www.britannica.com/EBchecked/topic/588969/tetracycline Lab 2: State the question/problem/objective:

The objective of this lab is to understand how to use a dichotomous key and characteristics of Algae and protists. A dichotomous key are basically keys which you answer another question which leads to the identification of Algea or what every was trying to be found. In this experiment a dichotomous key was used to find what Algae was found and protests, which is where, the they come in. To used the dichotomous key the characteristics must be identified. 

It was observed that the culture smelled like sour swamp water. The culture contained green flooting particles, there appears to be life forms on the top, the liquid is cloudy and brownish, there are brownish growths which are most likely mold. The reason why organism might differ from different parts is because some organisms may only be able to survive near plants or off them which is why it may be only found near plants or away from. One of the organisms were found in the culture was Chlamydomonas. Chlamydomonas fulfills all of the needs of life through different processes. It fulfills the energy through photosynthesis also through the nutrients being absorbed through the cell surface. The next part of being alive is cells this organism is a cell. To be alive information has to be present by having and eyespot it is able to sense light. A key part of being alive is reproduction, which they are involved in both asexual reproduction and sexual reproduction. The ability to adapt to an enlivenment as in evolution, which they seem to be successful with because there are about 500 different species of the Chlamydonas. If we left the hey infusion for another two months it would be come a more prevalent habitat meaning that there would be some organisms that did not survives because of the habitat and other organism would get stronger and more prevalent over time. The selective pressure that affects in the sample are natural selection. The selection of the fittest so if one organism need to live off something that is not available or is limited in the culture then it is highly likely that it will die out.

Record the specific steps you performed: The first part of the lab will be basically looking at a sample of a known organism under a microscope and characterizing it. The second part part of the lab is done by taking a sample from the culture and putting it on a wet mount and look at it under a microscope. Be sure to take samples from different parts and be sure to mark down where each sample was obtained. While looking at it under the microscope find organisms the measure and characterize the organisms. This should be repeated 2 times with each sample if you are in a group of three for a total of 6. The third part of the lab requires the jar to be shaken up then 10 mls of the culture and put it in a tub that contain 10 mls of sterile broth which is labeled 2 once added mix up and take 10mls out of the tub in to the next and repeat two more times. There should be four dilution by the end. After doing that you will be given 8 pieties dishes . Obtain four agar nutrient and four agar plus tetracycline plates. The tetracycline plates need to be labeled with “tet”. Label plates with 10-3,10-5,10-7, and 10-9. Make sure to mark the plates with group identifications. Each of the dilutions should be spread on there corresponding plates Include all of your raw data:

State Conclusions and future plans: The objective of this lab is to understand how to use a dichotomous key and characteristics of Algae and protists. We fulfilled both of these goals by taking samples from the transect and observing them under the microscope. Then using a dichotomous key to identify what we where looking at under the microscope. Though nothing went wrong many things could have including but not limited to mixing up the sample and josling your jar/culture. Something that would be interesting to see next time is to take samples from the culture a week later in the same spot and see if there was any change in the habitat in that sense. In conclusion this lab gave use the chance to learn how to use a dichotomous key to identify Algae and protists. Citations Chlamydomonas. (2014). In Encyclopaedia Britannica. Retrieved from http://www.britannica.com/EBchecked/topic/113450/Chlamydomonas

Lab 1: State the question / problem / objective: This lab was set up to help use understand natural selection and the characteristics of a niche in biotic and abiotic. This lab addresses these objectives through by observing and taking notes on a transact in nature and by us taking samples and setting them up thus allowing us to look at them next time. The topography of the of the transect is grass, rocks, cement and mud on a slight slant. 


Record the specific steps you performed: 1. To start the lab a 20 by 20 feet dimensions are measured for a transact which is marked by putting a popsicle stick in each corner. 2. Once the transact is layed out it should be noted the location to begin then topography among other obserations. 3. Observe and write down in a list the abiotic and biotic components in the transact. 4. Sample should be taken of the soil and ground vegetation using a sterile 50 ml conical tube. Its key to make sure to get reprehensive of the ground 5. Stay at the transact until the instructor has checked your work and said to go 6. 10 to 12 grams of the sample should be weighed out and placed in a plastic jar. The plastic jar should also contain 500 mls of deer park water. 7. Then add in 0.1gm of dried mild. Once added the jar should be gently mixed up for 10 seconds. 8. Once mixed the led should be removed and the jar should be placed in a safe place 9. Be sure to label the jar clearly.

Table 1 Evolutionary Specialization of Members of the Volvocine Line:

State Conclusions and future plans: The goal of this lab was to help use understand natural selection and the characteristics of a niche in biotic and abiotic. We where able to observe natural selection by looking at the animals that are still around . Biotic and abiotic organisms where observed by observing the transects and identifying what was abiotic and biotic. I don’t know how but if we did this experiment again and one of the goals was to understand natural selection then I would find away to make the goal more clearly understood. Though nothing went wrong many things could of including messing up the mixing of the jar. Something that would be interesting to see if we did is to take samples in different temperatures and weather conditions to see how that would change our results. The different variables would add more veriaty to the results thus giving us a better understanding of the life that the transect supports

Bentley/Walters-Conte/Zeller. (2014). Biological Life at AU. AU. Chlamydomonas. (2014). In Encyclopaedia Britannica. Retrieved from http://www.britannica.com/EBchecked/topic/113450/Chlamydomonas Nozaki, H. (1986). Sexual reproduction in gonium sociale (chlorophyta, volvocales). Phycologia, 25(1), 29-35. Retrieved from http://search.proquest.com/docview/14780095?accountid=8285 volvocid. (2014). In Encyclopaedia Britannica. Retrieved from http://www.britannica.com/EBchecked/topic/632667/volvocid Volvox. (2014). In Encyclopaedia Britannica. Retrieved from http://www.britannica.com/EBchecked/topic/632669/Volvox

2/9/14 File:AP edits Bio lab 2 210 draft 2.docx

Alyssa, please click the document within the image link. The reason why it's up so late (as with the previous lab) is because I did not know how to upload and link my documents to my notebook. If you want proof that I uploaded it earlier, then look up 'Hannah T Sherman' on openwetware. HS

'2/2/14 Please input the assignment that was due 1/31/14 by midnight. AP

File:AP edits Lad final draft write up for lab 1 Biological life at au (1).docx