User:Hattie Chung

Contact Info

Hattie Chung (an artistic interpretation)

• [Hattie Chung]
• [Massachusetts Institute of Technology]
• Cambridge, MA
• Email me through OpenWetWare

I am an undergraduate studying Biological Engineering at MIT. I've worked in the Langer Lab for the past two years on nucleic acid delivery and its immuno stimulatory effects. I learned about OWW (and BioBricks) through iGEM.

Education

• 2011, SB in Biological Engineering, MIT
• 2007, North Carolina School of Science and Mathematics

Research interests

1. Synthetic Biology and Cell Circuits
2. Nanotechnology and Nanomaterials
3. RNAi

Publications

1. [Engineering Novel Synthetic Biological Systems]
   • This is from my high school research project on generating electrical power using magnetotactic bacteria with the Duke iGEM team in summer 2006.

Useful links

• Introductory tutorial
• OpenWetWare help pages

Other Info

I love to travel. I spent last summer in Tanzania, last IAP in Korea, and this past summer in Tanzania and France. My favorite composers are Rachmaninov and Prokofiev; my favorite author is Isabel Allende.

Registration/Questionnaire: 20.109 Fall 2009

Last Name

Chung

First Name

Hattie

Preferred name

Hattie

Course/Minor

Course 20 Applied International Studies

Year of Graduation

2011

Telephone #

[919][961][4423]

Email

hchung11 AT mit DOT edu

Have you taken or are you taking...

7.05/5.07 (Biochemistry)
taken

7.06 (Cell Biology)
taking concurrently

7.02 (General Biology Lab)
no

5.310 (General Chemistry Lab)
no

Do you have any experience culturing cells (mammalian, yeast or microbial)?
yes: human mononuclear cells, mouse macrophage cells, mouse astrocytes, mouse neurons, bacteria

Do you have any experience in molecular biology (electrophoresis, PCR, etc)?
yes

Please briefly describe any previous laboratory experience

• Winter 2009: Asan Medical Research Center in Seoul, Korea
  • Rotated between a neuroscience lab and an endocrinology lab. Learned how to prepare and slice cryo-frozen samples for imaging. Learned how to harvest astrocytic cells from mice fetus.
• Fall 2007 - Spring 2009: MIT Langer Lab
  • Studied immuno-stimulatory effects of RNA delivery. Analyzed the relation between chemical structure of delivery agents and localization of payload release (i.e. stay in endosome or release in cytosol), which determines the function of small molecule RNA as immune-stimulatory or RNAi.
  • Technical tidbits: worked with human blood samples and isolating Peripheral Blood Mononuclear Cells (PBMCs), Human monocytic cells (THP-1), develop lipid library, uHPLCs, ZetaPals sizing and membrane potential measuring machine, FACS analysis, ELISAs
• Summer 2006: Duke University, intercollegiate Genetically Engineered Machines (iGEM)
  • Designed a microscale system to generate electric power using magnetotactic bacteria.
  • Technical tidbits: E.Coli cultures, PCR, soldering and fabricating coils with silicon wafers
• Summer 2005: Duke University, Wetsel Neuroscience and Endocrinology Laboratory
  • Studied pharmakinetics of various neurotransmitter receptor knockout mice strains that displayed schizophrenic and depressive behaviors.
  • Technical tidbits: PCR, Mouse subcutaneous injections, neuron potential reading in Norepinephrine Transporter Knockout mice, gel electrophoresis, Western blot

Anything else you would like us to know?

I just realized how labby I feel after rehashing my lab life...