User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/23

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(Autocreate 2013/05/23 Entry for User:Heather_N._Currey/Notebook/Taylor_C._elegans_Lab_Notebook)
Current revision (15:37, 23 May 2013) (view source)
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==Entry title==
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==23 May 2013==
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* Insert content here...
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*Streaked OP50-1 stocks for Adam and myself, put the excess inoculate in the refrigerator
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*Inoculated insulin clones for screen
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      -L4440: 10mL LB + 5µL carb + clone in a falcon tube, shaken overnight
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      -Ins-1: 10mL LB + 5µL carb + clone in a falcon tube, shaken overnight
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      -Ins-2: 10mL LB + 5µL carb + clone in a falcon tube, shaken overnight
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      -Ins-3: 10mL LB + 5µL carb + clone in a falcon tube, shaken overnight
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      -Ins-4: 10mL LB + 5µL carb + clone in a falcon tube, shaken overnight
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      -EACH WILL BE CONCENTRATED TO 1.5mL, I have 18 RNAi to use and will make 3-4 plates for each treatment
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Today I was only in the lab for the morning
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23 May 2013

  • Streaked OP50-1 stocks for Adam and myself, put the excess inoculate in the refrigerator
  • Inoculated insulin clones for screen
     -L4440: 10mL LB + 5µL carb + clone in a falcon tube, shaken overnight
     -Ins-1: 10mL LB + 5µL carb + clone in a falcon tube, shaken overnight
     -Ins-2: 10mL LB + 5µL carb + clone in a falcon tube, shaken overnight
     -Ins-3: 10mL LB + 5µL carb + clone in a falcon tube, shaken overnight
     -Ins-4: 10mL LB + 5µL carb + clone in a falcon tube, shaken overnight
     -EACH WILL BE CONCENTRATED TO 1.5mL, I have 18 RNAi to use and will make 3-4 plates for each treatment

Today I was only in the lab for the morning



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