User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/29

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(Autocreate 2013/05/29 Entry for User:Heather_N._Currey/Notebook/Taylor_C._elegans_Lab_Notebook)
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==Entry title==
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==29 May 2013==
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* Insert content here...
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*Seeded GFP/L4440 plates
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      -conc. from 10mL LB to 1.5mL
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      -blinded for adam: L4440=squiggles, GFP=wiggles
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*Performed restreak of GFP/L4440 plate using left over clone inoculate from plate seeding
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*was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth
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*Made M9 solution:
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      -Per 1 L of M9:
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            KH2PO4  3g
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            Na2HPO4  6g
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            NaCl      5g
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      Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)
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      add 1mL of MgSO4 sterily

Revision as of 18:19, 29 May 2013

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29 May 2013

  • Seeded GFP/L4440 plates
     -conc. from 10mL LB to 1.5mL
     -blinded for adam: L4440=squiggles, GFP=wiggles
  • Performed restreak of GFP/L4440 plate using left over clone inoculate from plate seeding
  • was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth
  • Made M9 solution:
     -Per 1 L of M9:
           KH2PO4   3g
           Na2HPO4   6g
           NaCl      5g
     Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)
     add 1mL of MgSO4 sterily 



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