User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/29

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(29 May 2013)
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*was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth
*was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth
*Made M9 solution:
*Made M9 solution:
-
       -Per 1 L of M9:
+
       Per 1 L of M9:               Adjusted for 700mL
-
 
+
      -KH2PO4  3g               2.1g
-
            KH2PO4  3g
+
      -Na2HPO4  6g            4.2g
-
            Na2HPO4  6g
+
      -NaCl      5g                 3.5g
-
             NaCl      5g
+
      -H2O to 1L                  water to 700mL
 +
      -read below                700µL MgSO4
       Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)
       Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)
       add 1mL of MgSO4 sterily  
       add 1mL of MgSO4 sterily  
 +
           

Revision as of 17:30, 29 May 2013

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29 May 2013

  • Seeded GFP/L4440 plates
     -conc. from 10mL LB to 1.5mL
     -blinded for adam: L4440=squiggles, GFP=wiggles
  • Performed restreak of GFP/L4440 plate using left over clone inoculate from plate seeding
  • was unable to perform egglay today due to inadequet numbers of healthy adult worms. Moved my stocks to the 25 degree incubator to accelerate their growth
  • Made M9 solution:
     Per 1 L of M9:               Adjusted for 700mL
     -KH2PO4   3g               2.1g
     -Na2HPO4   6g             4.2g
     -NaCl      5g                 3.5g
     -H2O to 1L                   water to 700mL
     -read below                 700µL MgSO4
     Autoclave bottle until it's cool to the touch (like what you do for NGM after you autoclave)
     add 1mL of MgSO4 sterily 
           



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