User:Helen L. Slucher/Notebook/CHEM-572/2014/02/11: Difference between revisions

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* Test the activity of citrate-HRP-GNPs using O2 binding assays
* Test the activity of citrate-HRP-GNPs using O2 binding assays


==Procedure==
Activity of citrate-HRP-GNPs
* We are going to use an assay provided by Dr. Hartings
**The assay uses a reagent that changes color when HRP is added in addition to hydrogen peroxide
**The hydrogen peroxide alone will change the color of the solution, so we will be comparing hydrogen peroxide alone with hydrogen peroxide and GNPs
*We also are going to run UV Vis of the samples to see how the absorption spectra changes over time
Dopamine Coating
#Dissolve dopamine in 100mL Tris Buffer to have a 10mM solution at pH=8.5
#Mix 17mL of GNP solution with 17mL of the dopamine solution
#Stir under air at room temperature for 1 hour
#Purify by centrifuging and then redisperse in water.
#Filtrate through syringe filters with cellulose acetate membranes with pore size of 1um
#Make 2 solutions.
## to be stored over night on lab bench
## to be stored over night in the refrigerator
==Notes==


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Revision as of 10:31, 11 February 2014

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Objective

  • Remake the dopamine addition from 02/05
  • Test the activity of citrate-HRP-GNPs using O2 binding assays

Procedure

Activity of citrate-HRP-GNPs

  • We are going to use an assay provided by Dr. Hartings
    • The assay uses a reagent that changes color when HRP is added in addition to hydrogen peroxide
    • The hydrogen peroxide alone will change the color of the solution, so we will be comparing hydrogen peroxide alone with hydrogen peroxide and GNPs
  • We also are going to run UV Vis of the samples to see how the absorption spectra changes over time

Dopamine Coating

  1. Dissolve dopamine in 100mL Tris Buffer to have a 10mM solution at pH=8.5
  2. Mix 17mL of GNP solution with 17mL of the dopamine solution
  3. Stir under air at room temperature for 1 hour
  4. Purify by centrifuging and then redisperse in water.
  5. Filtrate through syringe filters with cellulose acetate membranes with pore size of 1um
  6. Make 2 solutions.
    1. to be stored over night on lab bench
    2. to be stored over night in the refrigerator

Notes


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