User:Helen L. Slucher/Notebook/CHEM-572/2014/04/01

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==Goals==
==Goals==
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# Test UV/Vis of pellet from GNP spin down
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# Make beta method GNPs with all three proteins (HRP, hemoglobin, myoglobin)
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** Check to see if GNPs are there and look at size
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# Spin down gamma method HRP pellet
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# Spin down protein GNPs
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# Remake solutions so they are more concentrated
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** Take supernatant out and resuspend pellet
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==Notes: Kinetics==
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Reduction of heme group
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OPD, look for diamino...change of concentration over time, rates
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Change in absorbance = change in concentration
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Find molar absorptivity
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Deoxygen/oxygen bound
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Take off clear super, spin down until it doesn't show GNP activity

Revision as of 13:43, 1 April 2014

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Goals

  1. Make beta method GNPs with all three proteins (HRP, hemoglobin, myoglobin)
  2. Spin down gamma method HRP pellet
  3. Remake solutions so they are more concentrated

Notes: Kinetics

Reduction of heme group OPD, look for diamino...change of concentration over time, rates Change in absorbance = change in concentration Find molar absorptivity Deoxygen/oxygen bound Take off clear super, spin down until it doesn't show GNP activity



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