User:Helen L. Slucher/Notebook/CHEM 571/2013/08/28: Difference between revisions
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Citrate AuNP | Citrate AuNP | ||
Peak Wavelength | Peak Wavelength = 518 nm | ||
Absorbance at Peak | Absorbance at Peak = 0.612 | ||
Final Concentration | |||
<br>A<sub>450</sub>=0.389 | |||
Abs (518)/ Abs (450)= .612/ .389= 1.57 | Abs (518)/ Abs (450)= .612/ .389= 1.57 | ||
<br>A<sub>518</sub>/A<sub>450</sub>=1.57 | |||
Using the data found [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf here] | Using the data found [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf here]: | ||
<br>1.57 = 12 d/nm and a molar absorptivity of 1*10^8. | |||
<br>ε<sub>450</sub>=1.09*10<sup>8</sup> M<sup>-1</sup>cm<sup>-1</sup> | |||
<br>[[Image:Absorbtionvswavelength.png|right|]] | <br>[[Image:Absorbtionvswavelength.png|right|]] | ||
Beer's Law gives an equation for the absorption of light and the properties of the solution. | Beer's Law gives an equation for the absorption of light and the properties of the solution. | ||
:<math>\ A=\epsilon bc </math> | :<math>\ A=\epsilon bc </math> | ||
c=A<sub>450</sub>/ε<sub>450</sub> | |||
<br>c=3.57*10<sup>-9</sup> mol L<sup>-1</sup> | |||
<br>c=3. | |||
==References== | ==References== | ||
[http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 Dr. Harting's AU Biomaterials Design Lab] | [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28 Dr. Harting's AU Biomaterials Design Lab] |
Revision as of 15:43, 16 September 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
OverviewSynthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. BSA-Au Nanoparticles
Stock solutions made
Citrate-Au Nanoparticles
Stock solutions made
NotesCitrate AuNP Peak Wavelength = 518 nm Absorbance at Peak = 0.612 Final Concentration
Using the data found here:
Beer's Law gives an equation for the absorption of light and the properties of the solution.
c=A450/ε450
References |