User:Helen L. Slucher/Notebook/CHEM 571/2013/09/03: Difference between revisions
Line 8: | Line 8: | ||
==Overview== | ==Overview== | ||
* Determine the molar absorptivities of adenosine and inosine. | * Determine the molar absorptivities of adenosine and inosine. | ||
The data that we generate today will be important when we study adenosine deaminase (ADA), which converts adenosine to inosine. The difference between these two molecules is that adenosine contains a primary amine whereas inosine contains a carboxyl group. | *The data that we generate today will be important when we study adenosine deaminase (ADA), which converts adenosine to inosine. The difference between these two molecules is that adenosine contains a primary amine whereas inosine contains a carboxyl group. | ||
Adenosine and inosine have different absorption spectra. We will be observing changes in UV-Vis spectra to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. | *Adenosine and inosine have different absorption spectra. We will be observing changes in UV-Vis spectra to determine changes in concentration of both adenosine and inosine. In order to do this, we will need to know the molar absorptivity (ε) of both of these molecules. | ||
==Description== | ==Description== |
Revision as of 10:14, 12 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Overview
Description
Taking our Beer's Law relationship: (The darker the beer, the stronger the brew!) A=εbc where b is the path length (1cm), we can see that A is directly proportional to c and that if we plot A vs C, the slope will be ε.
DirectionsStock Solutions Adenosine has a molecular weight of 267.24g/mol; inosine has a molecular weight of 268.2g/mol.
RANDOM adenosine 0.25 x 10-5
Absorption Spectra Spectrum of each of your samples along with a blank Calibration Curve and Group work Do a least squares fit to the data and determine the slope of the line Determine the standard deviation for your data points. Determine the confidence interval for 90% and 95% confidence. Determine if any data can be ruled out using a Q-test. Unknown Determine the concentration of these unknowns from the calibration curves by propagating the error from the calibration curve to your concentration calculation. After making your calculation, find out what the calculation of the samples should be. CalculationsCalculations:
To dilute the solution of adenosine and inosine to the concentrations listed above:
Adenosine
Inosine
|