User:Helen L. Slucher/Notebook/CHEM 571/2013/09/17: Difference between revisions
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==Objective== | ==Objective== | ||
Determine the amount of reagent that is required to fully oxidize or fully reduce horseradish peroxidase. | |||
==Description== | ==Description== |
Revision as of 12:23, 12 October 2013
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ObjectiveDetermine the amount of reagent that is required to fully oxidize or fully reduce horseradish peroxidase. DescriptionIn order to obtain good results, we need our buffers to be as free of oxygen (oxygen is ... an oxidizing agent, so we need to try to remove it from our experiment) as we can get them. oxidation
reduction Follow along with the procedure for oxidation and, instead, use sodium dithionite for the reduction. Upon reduction the Soret peak will increase in intensity and shift to higher wavelengths. In order to prepare for tomorrow and have a better understanding of what we're doing today see the following references. This reference highlights some of the changes that we'll be observing in the spectra. Note, specifically, figure 4. We won't be using this exact experimental technique, tho. This reference goes more into detail into the kind of experiment we will be performing tomorrow. Note specifically the Redox Titrations portion of the Materials and Methods section.
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