User:Helen L. Slucher/Notebook/CHEM 571/2013/09/24: Difference between revisions
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Revision as of 14:39, 12 October 2013
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ObjectiveObserve the catalytic activity of pepsin in the presence and absence of pepstatin. This data will be compared with data we take in a later lab on pepsin-AuNPs. DescriptionWe will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE.
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