User:Helen L. Slucher/Notebook/CHEM 571/2013/09/25: Difference between revisions
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==Description== | ==Description== | ||
# Prepare the Gel and Assemble the Electrophoresis Cell | # Prepare the Gel and Assemble the Electrophoresis Cell | ||
## Remove comb and tape from the gels | ## Remove comb and tape from the gels | ||
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## Fill the inner and outer buffer chambers with running buffer | ## Fill the inner and outer buffer chambers with running buffer | ||
# Prepare and Load Samples | # Prepare and Load Samples | ||
## Heat your samples for 5 minutes at 100C (in the thermocycler) | ## Heat your samples for 5 minutes at 100C (in the thermocycler) | ||
## Load 20uL of protein ladder into column 1 of your gel | ## Load 20uL of protein ladder into column 1 of your gel |
Revision as of 14:44, 12 October 2013
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ObjectiveRun SDS-PAGE to observe amount of hemoglobin remaining after yesterday's digestion. Description
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