User:Helen L. Slucher/Notebook/CHEM 571/2013/10/01: Difference between revisions
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## luminol (use stock solution) | ## luminol (use stock solution) | ||
## 3-aminophthalic acid (product of reaction between luminol and H2O2 catalyzed by HRP. In order to take this measurement, react (in 1:1 ratio or with slight excess H2O2) luminol with H2O2 in presence of HRP. Allow the reaction to proceed for 5 minutes; take the spectrum) | ## 3-aminophthalic acid (product of reaction between luminol and H2O2 catalyzed by HRP. In order to take this measurement, react (in 1:1 ratio or with slight excess H2O2) luminol with H2O2 in presence of HRP. Allow the reaction to proceed for 5 minutes; take the spectrum) | ||
# Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer | |||
## Add HRP/Luminol stock solution to stopped flow mixer | |||
## Add H2O2 stock solution to stopped flow mixer | |||
## equilibrate mixer tubes with sample. | |||
## Initiate Mixing | |||
## Using luminol and 3-aminophthalic acid spectra as endpoints, determine the kinetics of 3-aminophthalic acid synthesis. | |||
==Data== | ==Data== |
Revision as of 15:27, 12 October 2013
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ObjectiveMonitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol. Description
Data
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