User:Helen L. Slucher/Notebook/CHEM 571/2013/10/02: Difference between revisions
From OpenWetWare
(Autocreate 2013/10/02 Entry for User:Helen_L._Slucher/Notebook/CHEM_571) |
|||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Objective== | ||
* Insert content here... | * Insert content here... | ||
==Procedure== | |||
# Chemiluminescence of luminol oxidation reaction initiated by stopped flow mixer | |||
## Add HRP/Luminol stock solution to stopped flow mixer | |||
## Add H2O2 stock solution to stopped flow mixer | |||
## equilibrate mixer tubes with sample. | |||
## Initiate Mixing | |||
## Measure light produced as result of reaction, integrated over a specific time range | |||
## Integrate area under the curve | |||
# Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer | |||
## Add HRP/Luminol stock solution to stopped flow mixer | |||
## Add H2O2 stock solution to stopped flow mixer | |||
## equilibrate mixer tubes with sample. | |||
## Initiate Mixing | |||
## Using luminol and 3-aminophthalic acid spectra as endpoints, determine the kinetics of 3-aminophthalic acid synthesis. | |||
Revision as of 14:52, 12 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
Procedure
|