User:Helen L. Slucher/Notebook/CHEM 571/2013/10/22: Difference between revisions

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==Entry title==
==Objective==
* Insert content here...
# Synthesize Pepsin-AuNPs for reactivity tests tomorrow
# Determine the Au:Protein ratio required for fiber formation for pepsin and HRP


==Description==
# Synthesis of HRP-AuNPs and Pepsin-AuNPs
## Into test tube that will contain a total of 5mL add
## Au solution such that the final concentration is 0.25mM
## Protein solution such that the final concentration reflects the following Au:Protein ratios
### 30
### 50
### 70
### 90
### 110
### 130
### 150
### 170
### 190
### 210
## Water up to 5mL
==Notes==
*Stock Solutions
# HRP: 15.3 μM
# Pepsin: 15.7 μM
# Gold: 2.52 mM
==Data==
*[[Image:Screen shot 2013-10-27 at 9.40.12 PM.png]]
*[[Image:Screen shot 2013-10-27 at 9.40.37 PM.png]]
*[[Image:Screen shot 2013-10-27 at 9.40.47 PM.png]]
*[[Image:Screen shot 2013-10-27 at 9.40.55 PM.png]]
*[[Image:Screen shot 2013-10-27 at 9.42.23 PM.png]]


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Revision as of 18:45, 27 October 2013

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Objective

  1. Synthesize Pepsin-AuNPs for reactivity tests tomorrow
  2. Determine the Au:Protein ratio required for fiber formation for pepsin and HRP

Description

  1. Synthesis of HRP-AuNPs and Pepsin-AuNPs
    1. Into test tube that will contain a total of 5mL add
    2. Au solution such that the final concentration is 0.25mM
    3. Protein solution such that the final concentration reflects the following Au:Protein ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
    4. Water up to 5mL

Notes

  • Stock Solutions
  1. HRP: 15.3 μM
  2. Pepsin: 15.7 μM
  3. Gold: 2.52 mM

Data


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